Document Detail

Specific detection of kappa light chain in uric acid stones.
MedLine Citation:
PMID:  9217284     Owner:  NLM     Status:  MEDLINE    
Proteins were extracted from uric acid stones with 6M guanidine chloride (pH 8.5), which were successively developed by 12% polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE). Amino acid sequence analysis of each band on SDS-PAGE revealed that major components in uric acid stones were immunoglobulin alpha heavy and kappa light chains. Although immunoglobulin heavy chain (gamma and mu, as well as alpha) and a kappa light chain were clearly detected in uric acid stones by Western blotting using their specific antibodies, no lambda chains whatsoever could be detected. The results suggest that immunoglobulins selectively containing kappa light chain might have specific functions in uric acid stone formation as stone matrices.
Y Hashimoto; K Kohri; Y Hayashi; A Moriyama; M Iguchi; K Asai; T Kato
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Life sciences     Volume:  61     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  1997  
Date Detail:
Created Date:  1997-08-04     Completed Date:  1997-08-04     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  249-53     Citation Subset:  IM    
Department of Urology, Nagoya City University Medical School, Mizuho-ku, Nagoya, Japan.
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MeSH Terms
Amino Acid Sequence
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Hemoglobin A / chemistry
Immunoglobulin Heavy Chains / chemistry
Immunoglobulin kappa-Chains / analysis*,  chemistry
Molecular Sequence Data
Sequence Homology
Uric Acid / analysis*
Urinary Calculi / chemistry*,  immunology*
Reg. No./Substance:
0/Guanidines; 0/Immunoglobulin Heavy Chains; 0/Immunoglobulin kappa-Chains; 113-00-8/Guanidine; 69-93-2/Uric Acid; 9034-51-9/Hemoglobin A

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