Document Detail

Specific detection and differentiation of Ochrobactrum anthropi, Ochrobactrum intermedium and Brucella spp. by a multi-primer PCR that targets the recA gene.
MedLine Citation:
PMID:  18065669     Owner:  NLM     Status:  MEDLINE    
Ochrobactrum anthropi, Ochrobactrum intermedium and Brucella spp. are phenotypically and genetically closely related pathogens that may cause disease with similar clinical presentation. Consequently, difficulties in their identification and differentiation have been reported. In this study, a sensitive recA gene-based multi-primer single-target PCR (MP-ST-PCR) was developed that allowed the specific detection and differentiation of these clinically relevant pathogens. The specificity of the assay was evaluated using a representative panel of 50 O. anthropi and 16 O. intermedium strains and the type strains of all Brucella spp. Detection limits for purified DNA from O. anthropi, O. intermedium and Brucella melitensis were 100, 10 and 100 fg, respectively. Brucella DNA was also successfully detected in various clinical specimens from a human patient with culture-proven brucellosis and from a Brucella-infected sheep and its aborted fetuses. The sensitivity of the MP-ST-PCR was comparable to that of an evaluated in-house Brucella real-time PCR assay. The developed assay closes a diagnostic gap and provides a simple but robust tool for the sensitive detection and correct identification of O. anthropi, O. intermedium and Brucella spp.
Holger Christian Scholz; Martin Pfeffer; Angela Witte; Heinrich Neubauer; Sascha Al Dahouk; Ulrich Wernery; Herbert Tomaso
Related Documents :
11162079 - Real-time detection of brucella abortus, brucella melitensis and brucella suis.
21967769 - Xrcc1 suppresses somatic hypermutation and promotes alternative nonhomologous end joini...
17124719 - Real time pcr profiling of 330 human micro-rnas.
20112879 - Application of quantitative real-time pcr for quantification of rhodococcus sp. eh831 i...
16785629 - Real-time quantitative polymerase chain reaction analysis of mitochondrial dna point mu...
19198529 - Rapid detection of wuchereria bancrofti and brugia malayi in mosquito vectors (diptera:...
18970189 - Surface-functionalized silica-coated gold nanoparticles and their bioapplications.
2408039 - Genomic subtraction for cloning dna corresponding to deletion mutations.
19562789 - Precisely programmed and robust 2d streptavidin nanoarrays by using periodical nanomete...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of medical microbiology     Volume:  57     ISSN:  0022-2615     ISO Abbreviation:  J. Med. Microbiol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-10     Completed Date:  2008-05-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0224131     Medline TA:  J Med Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  64-71     Citation Subset:  IM    
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D-80937 Munich, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacterial Typing Techniques*
Brucella / genetics,  isolation & purification*
Brucellosis / diagnosis
DNA Primers
DNA, Bacterial / analysis*
Gram-Negative Bacterial Infections / diagnosis,  microbiology*
Ochrobactrum / genetics,  isolation & purification*
Ochrobactrum anthropi / genetics,  isolation & purification
Polymerase Chain Reaction
Rec A Recombinases / genetics*
Sensitivity and Specificity
Reg. No./Substance:
0/DNA Primers; 0/DNA, Bacterial; EC 2.7.7.-/Rec A Recombinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Molecular typing of Japanese Escherichia coli O157 : H7 isolates from clinical specimens by multiloc...
Next Document:  Identification and characterization of a vancomycin-resistant Staphylococcus aureus isolated from Ko...