Document Detail


Specific detection and differentiation of Ochrobactrum anthropi, Ochrobactrum intermedium and Brucella spp. by a multi-primer PCR that targets the recA gene.
MedLine Citation:
PMID:  18065669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ochrobactrum anthropi, Ochrobactrum intermedium and Brucella spp. are phenotypically and genetically closely related pathogens that may cause disease with similar clinical presentation. Consequently, difficulties in their identification and differentiation have been reported. In this study, a sensitive recA gene-based multi-primer single-target PCR (MP-ST-PCR) was developed that allowed the specific detection and differentiation of these clinically relevant pathogens. The specificity of the assay was evaluated using a representative panel of 50 O. anthropi and 16 O. intermedium strains and the type strains of all Brucella spp. Detection limits for purified DNA from O. anthropi, O. intermedium and Brucella melitensis were 100, 10 and 100 fg, respectively. Brucella DNA was also successfully detected in various clinical specimens from a human patient with culture-proven brucellosis and from a Brucella-infected sheep and its aborted fetuses. The sensitivity of the MP-ST-PCR was comparable to that of an evaluated in-house Brucella real-time PCR assay. The developed assay closes a diagnostic gap and provides a simple but robust tool for the sensitive detection and correct identification of O. anthropi, O. intermedium and Brucella spp.
Authors:
Holger Christian Scholz; Martin Pfeffer; Angela Witte; Heinrich Neubauer; Sascha Al Dahouk; Ulrich Wernery; Herbert Tomaso
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of medical microbiology     Volume:  57     ISSN:  0022-2615     ISO Abbreviation:  J. Med. Microbiol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-10     Completed Date:  2008-05-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0224131     Medline TA:  J Med Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  64-71     Citation Subset:  IM    
Affiliation:
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D-80937 Munich, Germany. Holger1Scholz@bundeswehr.org
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Typing Techniques*
Brucella / genetics,  isolation & purification*
Brucellosis / diagnosis
DNA Primers
DNA, Bacterial / analysis*
Gram-Negative Bacterial Infections / diagnosis,  microbiology*
Humans
Ochrobactrum / genetics,  isolation & purification*
Ochrobactrum anthropi / genetics,  isolation & purification
Polymerase Chain Reaction
Rec A Recombinases / genetics*
Sensitivity and Specificity
Sheep
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Bacterial; EC 2.7.7.-/Rec A Recombinases

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