Document Detail

Specific detection by the polymerase chain reaction of potentially allergenic salmonid fish residues in processed foods.
MedLine Citation:
PMID:  22738970     Owner:  NLM     Status:  In-Data-Review    
Salmonid fish is one of the allergenic items that are recommended to be labeled in the Japanese allergen-labeling system. This study develops a salmonid-specific polymerase chain reaction (PCR) method. A new primer pair, SKE-F/SKE-R, was designed to specifically detect the salmonid fish gene encoding mitochondrial DNA cytochrome b. Genomic DNAs extracted from 58 kinds of seafood and 11 kinds of processed food were individually subjected to PCR by using the primer pair, and a salmonid-specific fragment of 212 bp was only amplified in the salmonid samples and salmonid-containing processed foods. The detection limit of the PCR method was as low as 0.02 fg/µL of salmonid fish DNA (corresponding to 10 copies). There is no ELISA method for salmonid fish, making our PCR method the only reliable measure for detecting salmonid fish in processed foods.
Shoichiro Ishizaki; Yumiko Sakai; Takeo Yano; Shigeru Nakano; Toshihiro Yamada; Yuji Nagashima; Kazuo Shiomi; Yoshiki Nakao; Hiroshi Akiyama
Publication Detail:
Type:  Journal Article     Date:  2012-05-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  76     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-06-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  980-5     Citation Subset:  IM    
Tokyo University of Marine Science and Technology.
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