Document Detail

Specific conversion of D-galactose into D-galacturonic acid residues in glycoproteins: a facile method for carbohydrate linkage-analysis.
MedLine Citation:
PMID:  709566     Owner:  NLM     Status:  MEDLINE    
The terminal D-galactopyranosyl residues of asialoglycopeptides isolated from human alpha1-acid glycoprotein were oxidized in nearly quantitative yield to the corresponding uronic acid residues by a two-step sequence employing D-galactose oxidase followed by treatment with Tollens reagent, Ag(NH3)+2. Mild acid hydrolysis of the oxidized glycopeptides led to the isolation of the corresponding aldobiuronic acid(s). Structural and colorimetric analysis revealed that only one aldobiuronic acid 2-amino-2-deoxy-4-O-(beta-D-galactopyranosyluronic acid)-D-glucose, was isolated from the oxidized glycopeptides of alpha1-acid glycoprotein. This method can readily distinguish between the (1 leads to 3), (1 leads to 4), and (1 leads to 6) isomers of the corresponding aldobiuronic acids.
J S Myers; O Gabriel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Carbohydrate research     Volume:  67     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  1978 Nov 
Date Detail:
Created Date:  1979-01-15     Completed Date:  1979-01-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  223-34     Citation Subset:  IM    
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MeSH Terms
Chemical Phenomena
Glycopeptides / analysis
Orosomucoid / analysis*
Uronic Acids*
Reg. No./Substance:
0/Glycopeptides; 0/Orosomucoid; 0/Uronic Acids; 26566-61-0/Galactose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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