| Specific conversion of D-galactose into D-galacturonic acid residues in glycoproteins: a facile method for carbohydrate linkage-analysis. | |
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MedLine Citation:
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PMID: 709566 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The terminal D-galactopyranosyl residues of asialoglycopeptides isolated from human alpha1-acid glycoprotein were oxidized in nearly quantitative yield to the corresponding uronic acid residues by a two-step sequence employing D-galactose oxidase followed by treatment with Tollens reagent, Ag(NH3)+2. Mild acid hydrolysis of the oxidized glycopeptides led to the isolation of the corresponding aldobiuronic acid(s). Structural and colorimetric analysis revealed that only one aldobiuronic acid 2-amino-2-deoxy-4-O-(beta-D-galactopyranosyluronic acid)-D-glucose, was isolated from the oxidized glycopeptides of alpha1-acid glycoprotein. This method can readily distinguish between the (1 leads to 3), (1 leads to 4), and (1 leads to 6) isomers of the corresponding aldobiuronic acids. |
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Authors:
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J S Myers; O Gabriel |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Carbohydrate research Volume: 67 ISSN: 0008-6215 ISO Abbreviation: Carbohydr. Res. Publication Date: 1978 Nov |
Date Detail:
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Created Date: 1979-01-15 Completed Date: 1979-01-15 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 223-34 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chemical Phenomena Chemistry Galactose* Glycopeptides / analysis Humans Orosomucoid / analysis* Oxidation-Reduction Uronic Acids* |
| Chemical | |
Reg. No./Substance:
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0/Glycopeptides; 0/Orosomucoid; 0/Uronic Acids; 26566-61-0/Galactose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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