Document Detail


Specific chromomeres on the chicken W lampbrush chromosome contain specific repetitive DNA sequence families.
MedLine Citation:
PMID:  9688523     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromomeres 1 and 3 of the chicken W lampbrush chromosome contain most of the EcoRI and XhoI repeat sequence families respectively. These chromomeres were stained with DAPI and their sizes relative to other W chromomeres were observed. Their relative contents of EcoRI and XhoI repeats were determined using fluorescence in situ hybridization with genomic probes for each of the two repeat families. There were two types of W chromosome in the chickens (White Leghorn and Rhode Island Red) used in this study with respect to the amount of EcoRI repeat. A high-copy-number type has about 4000 copies of the 1.2-kb repeat per genome and shows a large fluorescence signal on W chromomere 1. A low-copy-number type has about 700 copies per genome and does not have a detectable chromomere 1 on W chromosome, nor does it show FISH labelling in the region normally occupied by chromomere 1. The genome of Fayoumi chickens has about one-sixth the amount of the XhoI sequence family of White Leghorns. W lampbrush chromomere 3 is much smaller and its FISH labelling with the XhoI probe is much weaker in Fayoumis than in White Leghorns. These results demonstrate that in the chicken W chromosome, specific chomomeres are occupied by specific DNA repeat sequence families.
Authors:
I Solovei; A Ogawa; M Naito; S Mizuno; H Macgregor
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  6     ISSN:  0967-3849     ISO Abbreviation:  Chromosome Res.     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-09-23     Completed Date:  1998-09-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  323-7     Citation Subset:  IM    
Affiliation:
Institute of Anthropology and Human Genetics, LMU, München, Germany. Irina.Solovei@lrz.uni-muenchen.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Cells
Blotting, Southern
Chickens / genetics*
Chromosomes / genetics*
DNA Probes
DNA Restriction Enzymes
Female
Fluorescent Dyes
Genetic Markers*
Heterochromatin / genetics*
In Situ Hybridization, Fluorescence
Indoles
Multigene Family
Repetitive Sequences, Nucleic Acid*
Chemical
Reg. No./Substance:
0/DNA Probes; 0/Fluorescent Dyes; 0/Genetic Markers; 0/Heterochromatin; 0/Indoles; 47165-04-8/DAPI; EC 3.1.21.-/DNA Restriction Enzymes

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