Document Detail


Specific binding of plasminogen to vitronectin. Evidence for a modulatory role of vitronectin on fibrin(ogen)-induced plasmin formation by tissue plasminogen activator.
MedLine Citation:
PMID:  1693270     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vitronectin immobilized onto polystyrene microtiter wells was demonstrated to specifically bind plasminogen in a concentration-dependent manner, yielding an estimated KD = 0.4 microM. Heparin only moderately interfered with the vitronectin-plasminogen interaction, whereas high concentrations of 6-amino-hexanoic acid inhibited binding. Utilizing a ligand-blotting procedure in which plasminogen was reacted with proteolytic fragments of vitronectin, transblotted onto nitrocellulose, the plasminogen-binding site of vitronectin was localized to the heparin-binding domain of the adhesive protein. Moreover, vitronectin was found to inhibit in a dose-dependent fashion the fibrin(ogen)-induced activation of plasminogen by tissue plasminogen activator. These results provide the first evidence for a novel vitronectin-mediated control of plasminogen activation potentially relevant for directional clot-lysis and plasmin-dependent proteolysis in extracellular matrices.
Authors:
K T Preissner
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  168     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1990 May 
Date Detail:
Created Date:  1990-07-05     Completed Date:  1990-07-05     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  966-71     Citation Subset:  IM    
Affiliation:
Clinical Research Unit for Blood Coagulation and Thrombosis, Max-Planck-Gesellschaft, Giessen, F.R.G.
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MeSH Terms
Descriptor/Qualifier:
Electrophoresis, Polyacrylamide Gel
Enzyme Activation
Fibrin / antagonists & inhibitors,  physiology*
Fibrinolysin / biosynthesis*
Glycoproteins / metabolism*
Humans
Peptide Fragments / metabolism
Plasminogen / metabolism*
Protein Binding
Tissue Plasminogen Activator / physiology*
Vitronectin
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Peptide Fragments; 0/Vitronectin; 9001-31-4/Fibrin; 9001-91-6/Plasminogen; EC 3.4.21.68/Tissue Plasminogen Activator; EC 3.4.21.7/Fibrinolysin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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