| Specific binding of plasminogen to vitronectin. Evidence for a modulatory role of vitronectin on fibrin(ogen)-induced plasmin formation by tissue plasminogen activator. | |
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MedLine Citation:
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PMID: 1693270 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vitronectin immobilized onto polystyrene microtiter wells was demonstrated to specifically bind plasminogen in a concentration-dependent manner, yielding an estimated KD = 0.4 microM. Heparin only moderately interfered with the vitronectin-plasminogen interaction, whereas high concentrations of 6-amino-hexanoic acid inhibited binding. Utilizing a ligand-blotting procedure in which plasminogen was reacted with proteolytic fragments of vitronectin, transblotted onto nitrocellulose, the plasminogen-binding site of vitronectin was localized to the heparin-binding domain of the adhesive protein. Moreover, vitronectin was found to inhibit in a dose-dependent fashion the fibrin(ogen)-induced activation of plasminogen by tissue plasminogen activator. These results provide the first evidence for a novel vitronectin-mediated control of plasminogen activation potentially relevant for directional clot-lysis and plasmin-dependent proteolysis in extracellular matrices. |
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Authors:
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K T Preissner |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 168 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1990 May |
Date Detail:
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Created Date: 1990-07-05 Completed Date: 1990-07-05 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 966-71 Citation Subset: IM |
Affiliation:
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Clinical Research Unit for Blood Coagulation and Thrombosis, Max-Planck-Gesellschaft, Giessen, F.R.G. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Electrophoresis, Polyacrylamide Gel Enzyme Activation Fibrin / antagonists & inhibitors, physiology* Fibrinolysin / biosynthesis* Glycoproteins / metabolism* Humans Peptide Fragments / metabolism Plasminogen / metabolism* Protein Binding Tissue Plasminogen Activator / physiology* Vitronectin |
| Chemical | |
Reg. No./Substance:
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0/Glycoproteins; 0/Peptide Fragments; 0/Vitronectin; 9001-31-4/Fibrin; 9001-91-6/Plasminogen; EC 3.4.21.68/Tissue Plasminogen Activator; EC 3.4.21.7/Fibrinolysin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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