Document Detail

Specific antibody responses against Neospora caninum recombinant rNcGRA7, rNcSAG4, rNcBSR4 and rNcSRS9 proteins are correlated with virulence in mice.
MedLine Citation:
PMID:  23343779     Owner:  NLM     Status:  Publisher    
SUMMARY The intraspecific diversity of Neospora caninum is a determinant for in vivo parasite virulence and in vitro parasite behaviour. The relationship between isolate virulence and specific antibody responses against key parasite proteins has not been well characterized. The response kinetics and the differences in specific anti-rNcGRA7, -rNcSAG4, -rNcBSR4 and -rNcSRS9 antibody levels were analysed by recombinant protein-based ELISA in groups of mice inoculated with 10 different N. caninum isolates that differ in their virulence. The majority of the virulence parameters analysed correlated with the specific antibody levels against the 4 recombinant proteins. The antibodies developed against the highly immunogenic protein NcGRA7 were significantly higher in mice inoculated with high virulence isolates than in those inoculated with low-to-moderate virulence isolates in both non-pregnant and pregnant mouse models. Moreover, these levels were correlated with the anti-N. caninum IgG1 and IgG2a responses and the in vitro tachyzoite yield at 56 h. The antibodies directed against the bradyzoite-specific proteins were not detected in a non-pregnant mouse model. However, some seropositive mice were found in groups inoculated with high virulence isolates in a pregnant mouse model. NcGRA7 and NcSAG4 are proteins clearly correlated with virulence, and to a lesser extent NcBSR4 and NcSRS9 proteins. Moreover, antibodies to bradyzoite-specific proteins appear to also be related to virulence in mice. Further analyses should be performed in order to verify the usefulness of these proteins as predictive markers for virulence in an experimental bovine model of neosporosis.
Elena Jiménez-Ruiz; Gregori Bech-Sàbat; Gema Alvarez-García; Javier Regidor-Cerrillo; Laura Hinojal-Campaña; Luis M Ortega-Mora
Related Documents :
15175079 - Disturbed trafficking of dystrophin and associated proteins in targetoid phenomena afte...
2461699 - The effect of chronic ethanol ingestion on protein metabolism in type-i- and type-ii-fi...
19305149 - Quantitation of selective autophagic protein aggregate degradation in vitro and in vivo...
7083779 - Interpretation of tracer studies on plasma protein turnover: comparison of methods and ...
24686059 - A fibronectin-binding protein of borrelia hermsii expressed in the blood of mice with r...
9429159 - Phosphorylation of a high molecular weight (approximately 600 kda) protein regulates ca...
21905219 - Gel-free proteomic identification of the bacillus subtilis insoluble spore coat protein...
15149869 - Phagocyte-specific calcium-binding s100 proteins as clinical laboratory markers of infl...
24305859 - Inheritance of seed protein content and other agronomic characters in long bean (vigna ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-24
Journal Detail:
Title:  Parasitology     Volume:  -     ISSN:  1469-8161     ISO Abbreviation:  Parasitology     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401121     Medline TA:  Parasitology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1-11     Citation Subset:  -    
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Relationships Among Neurocognition, Symptoms, and Functioning in Treatment-Resistant Depression.
Next Document:  Characterization of Candidate Anti-Allergic Probiotic Strains in a Model of Th2-Skewed Human Periphe...