Document Detail

Specific and Rapid Quantification of Viable Listeria monocytogenes Using Fluorescence in situ Hybridization in Combination with Filter Cultivation.
MedLine Citation:
PMID:  21566384     Owner:  NLM     Status:  In-Data-Review    
We developed a new method that rapidly and specifically enumerates only viable Listeria monocytogenes in food using fluorescence in situ hybridization in combination with filter cultivation (FISHFC). Viable L. monocytogenes could be specifically quantified within 16 h using an Alexa647-labeled mRL-2 probe. The coefficient of the correlation between the new method and the conventional plating method was 0.959.
Ikufumi Fuchizawa; Shigemasa Shimizu; Masashi Ootsubo; Yuji Kawai; Koji Yamazaki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microbes and environments / JSME     Volume:  24     ISSN:  1342-6311     ISO Abbreviation:  Microbes Environ.     Publication Date:  2009  
Date Detail:
Created Date:  2011-05-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9710937     Medline TA:  Microbes Environ     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  273-5     Citation Subset:  -    
Division of Marine Life Science, Faculty of Fisheries Sciences, Hokkaido University.
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