Document Detail


Specific HR-associated recognition of secreted proteins from Cladosporium fulvum occurs in both host and non-host plants.
MedLine Citation:
PMID:  10998185     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The resistance of tomato (Lycopersicon esculentum) to the pathogenic fungus Cladosporium fulvum complies with the gene-for-gene concept. Host resistance is based on specific recognition of extracellular fungal proteins, resulting in a hypersensitive response (HR). Five proteins secreted by C. fulvum were purified and the encoding cDNA clone was obtained from two novel ones among them. Various tomato breeding lines and accessions of Lycopersicon pimpinellifolium were tested for their recognitional specificity by injection of the purified proteins or potato virus X-based expression of the cDNA. We found that HR-associated recognition of one or more of these proteins, in addition to recognition of the race-specific elicitors AVR4 and AVR9 of C. fulvum, occurs among Lycopersicon species. Studies on the inheritance of this recognition confirmed that single dominant genes are involved. Furthermore, one of the extracellular proteins of C. fulvum is specifically recognized by Nicotiana paniculata, which is not a host for C. fulvum. These results indicate that plants have a highly effective surveillance system for the presence of 'foreign' proteins, which, together with the high mutation rate of pathogens, can explain the complex gene-for-gene relationships frequently observed in pathosystems.
Authors:
R Laugé; P H Goodwin; P J de Wit; M H Joosten
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  23     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-12-06     Completed Date:  2000-12-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  735-45     Citation Subset:  IM    
Affiliation:
Laboratory of Phytopathology, Wageningen University, Binnenhaven 9, 6709 PD Wageningen, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Cladosporium / metabolism*
DNA, Complementary
DNA, Fungal
Fungal Proteins / genetics,  metabolism*
Lycopersicon esculentum / microbiology
Molecular Sequence Data
Open Reading Frames
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/DNA, Fungal; 0/ECP4 protein, Cladosporium fulvum; 0/ECP5 protein, Cladosporium fulvum; 0/Fungal Proteins

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