Document Detail


Specific DNA Duplex Formation at an Artificial Lipid Bilayer: towards a New DNA Biosensor Technology.
MedLine Citation:
PMID:  22344904     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A novel technique is described which comprises a base-specific DNA duplex formation at a lipid bilayerH(2) O-phase boundary layer. Two different probes of oligonucleotides both carrying a double-tailed lipid at the 5'-terminus were incorporated into stable artificial lipid bilayers separating two compartments (cis/trans-channel) of an optically transparent microfluidic sample carrier with perfusion capabilities. Both the cis- and trans-channels are filled with saline buffer. Injection of a cyanine-5-labeled target DNA sequence, which is complementary to only one of the oligonucleotide probes, into the cis-channel, followed by a thorough perfusion, leads to an immobilization of the labeled complementary oligonucleotide on the membrane as detected by single-molecule fluorescence spectroscopy and microscopy. In the case of fluorescent but non-complementary DNA sequences, no immobilized fluorescent oligonucleotide duplex could be detected on the membrane. This clearly verifies a specific duplex formation at the membrane interface.
Authors:
Emma Werz; Sergei Korneev; Malayko Montilla-Martinez; Richard Wagner; Roland Hemmler; Claudius Walter; Jörg Eisfeld; Karsten Gall; Helmut Rosemeyer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chemistry & biodiversity     Volume:  9     ISSN:  1612-1880     ISO Abbreviation:  Chem. Biodivers.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197449     Medline TA:  Chem Biodivers     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  272-81     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Verlag Helvetica Chimica Acta AG, Zürich.
Affiliation:
Organic Chemistry I - Bioorganic Chemistry, Institute of Chemistry, University of Osnabrück, Barbarastrasse 7, D-49069 Osnabrück.
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