Document Detail

Species variation of gonadotropic activity in an in vitro assay measuring androgen formation by carp (Cyprinus carpio) testis with special reference to bioassay of piscine gonadotropins.
MedLine Citation:
PMID:  3770434     Owner:  NLM     Status:  MEDLINE    
The objectives of this study were to establish a suitable and validated in vitro bioassay of piscine gonadotropins (GTHs) by using a carp testis androgen production system and to compare the androgenic responses in such an assay to gonadotropins from various vertebrate species. The testes from mature carp with gonadosomatic indices of 8-30% were used. Androgen production was first compared with respect to methods for preparation of the carp testis (sliced, minced, homogenized, and collagenase-dispersed testis preparations). The time course of androgen formation, the effects of xanthine and theophylline, and other factors on androgen production also were investigated. Theophylline was more effective than xanthine in potentiation of gonadotropin-evoked androgen formation by carp testis. The testis preparations were incubated in medium 199 (pH 7.40) containing 2 mM theophylline with shaking at 100 cycles/min at 25 degrees C for 4 hr. Homogenized testis preparations had limited ability for androgen production, while sliced, minced, and minced-collagenase-dispersed testis preparations were highly responsive to gonadotropins for androgen production. The minced testis preparation, utilizing 100 mg/ml incubation medium per vial, was chosen as the standard incubation procedure in this study. The minced testis androgen production assay was highly sensitive to gonadotropins from several piscine species (silver carp, common carp, and salmon), and all these GTHs produced parallel dose-related androgen production curves. Mammalian GTHs were also capable of promoting androgen formation by carp testis, but they were much less potent than were piscine GTHs. Pregnant mares' serum gonadotropin (PMSG) was more effective than human chorionic gonadotropin (hCG) in evoking carp testis androgen production.(ABSTRACT TRUNCATED AT 250 WORDS)
J Y Yu; T M Lin
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  General and comparative endocrinology     Volume:  62     ISSN:  0016-6480     ISO Abbreviation:  Gen. Comp. Endocrinol.     Publication Date:  1986 Jun 
Date Detail:
Created Date:  1986-12-12     Completed Date:  1986-12-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370735     Medline TA:  Gen Comp Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  428-36     Citation Subset:  IM    
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MeSH Terms
Androgens / biosynthesis*
Biological Assay
Carps / metabolism*
Cyprinidae / metabolism*
Gonadotropins / metabolism*
Species Specificity
Testis / metabolism*
Reg. No./Substance:
0/Androgens; 0/Gonadotropins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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