Document Detail


Species specificity of iron delivery in hybridomas.
MedLine Citation:
PMID:  3372446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies with Human X Human (H X H), Human X Mouse (H X M), and Mouse X Mouse (M X M) hybridomas have enabled us to define specific factors that affect hybridoma growth in a species-specific manner. Three transferrins and three lipophilic iron chelates have been tested for their ability to support hybridoma proliferation and antibody production. The results of these studies demonstrate that H X H hybridomas do not respond to bovine transferrin a+ concentrations up to 100 micrograms/ml and are approximately 100-fold less responsive to mouse transferrin than to human transferrin. H X M and M X M hybridomas respond equally to human or mouse transferrin but are 100-fold less sensitive to bovine transferrin. An antibody to the human transferrin receptor inhibited the growth-promoting activity of human or mouse transferrin on H X H hybridomas but was ineffective on H X M hybridomas. This demonstrated the functionality of the human transferrin receptor in H X H hybridomas and that human, mouse, and bovine transferrin were interacting through the mouse transferrin receptor in H X M hybridomas. H X H and H X M hybridomas respond similarly to three different iron chelates exhibiting 80 to 110% of the growth response to human transferrin. M X M hybridomas fail to respond to the iron chelates at similar concentrations, suggesting that the human genome present in the other hybridoma species confers a unique ability for utilizing iron when delivered in this form.
Authors:
C R Ill; T Brehm; B K Lydersen; R Hernandez; K G Burnett
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  In vitro cellular & developmental biology : journal of the Tissue Culture Association     Volume:  24     ISSN:  0883-8364     ISO Abbreviation:  In Vitro Cell. Dev. Biol.     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-07-08     Completed Date:  1988-07-08     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8506951     Medline TA:  In Vitro Cell Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  413-9     Citation Subset:  IM    
Affiliation:
Department of In Vitro Antibody Production, Hybritech, Inc., San Diego, California 92121.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cell Line
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Humans
Hybridomas / metabolism*
Iron / metabolism*
Mice
Receptors, Transferrin / metabolism
Species Specificity
Transferrin / pharmacology
Chemical
Reg. No./Substance:
0/Receptors, Transferrin; 11096-37-0/Transferrin; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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