Document Detail


Species-specific residues calibrate SoxR sensitivity to redox-active molecules.
MedLine Citation:
PMID:  23205737     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In enterics, the transcription factor SoxR triggers a global stress response by sensing a broad spectrum of redox-cycling compounds. In the non-enteric bacteria Pseudomonas aeruginosa and Streptomyces coelicolor, SoxR is activated by endogenous redox-active small molecules and only regulates a small set of genes. We investigated if the more general response in enterics is reflected in the ability of SoxR to sense a wider range of redox-cycling compounds. Indeed, while Escherichia coli SoxR is tuned to structurally diverse compounds that span a redox range of -450 to +80 mV, P. aeruginosa and S. coelicolor SoxR are less sensitive to viologens, which have redox potentials below -350 mV. Using a mutagenic approach, we pinpointed three amino acids that contribute to the reduced sensitivity of P. aeruginosa and S. coelicolor SoxR. Notably these residues are not conserved in homologues of the Enterobacteriaceae. We further identified a motif within the sensor domain that tunes the activity of SoxR from enterics - inhibiting constitutive activity while allowing sensitivity to drugs with low redox potentials. Our findings highlight how small alterations in structure can lead to the evolution of proteins with distinct specificities for redox-active small molecules.
Authors:
Rebecca Sheplock; David A Recinos; Natalie Mackow; Lars E P Dietrich; Monica Chander
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-12-04
Journal Detail:
Title:  Molecular microbiology     Volume:  87     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-14     Completed Date:  2013-06-21     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  368-81     Citation Subset:  IM    
Copyright Information:
© 2012 Blackwell Publishing Ltd.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism*
DNA Mutational Analysis
Escherichia coli / drug effects,  metabolism*
Oxidants / metabolism*
Oxidation-Reduction
Pseudomonas aeruginosa / drug effects,  metabolism*
Streptomyces coelicolor / drug effects,  metabolism*
Transcription Factors / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
R15 GM093366/GM/NIGMS NIH HHS; R15GM093366/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Oxidants; 0/Transcription Factors; 137804-81-0/SoxR protein, Bacteria
Comments/Corrections

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