Document Detail


Species identification of blood and bloodstains by enzyme-linked immunosorbent assay (ELISA) using anti-human immunoglobulin kappa light chain monoclonal antibody.
MedLine Citation:
PMID:  2494103     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A series of experiments was conducted to establish a species identification method by means of the enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody with high specificity, the result of which is reported. The limit of human IgG detection by inhibition ELISA using mouse anti-human immunoglobulin kappa light chain (Ig kappa) monoclonal antibody is 0.01 microgram. Using this method it was possible to detect human Ig kappa from bloodstains in dilutions up to 1:100,000 (dilution multiple of bloodstain extract to the original amount of blood). Human Ig kappa was detectable in human bloodstains in dilutions up to 1:50,000 one year after stain preparation and in dilutions up to 1:10,000 4 years after stain preparation, showing that this method is applicable to species identification of old bloodstains. When primates other than human were studied for specificity, red-chested tamarin and Japanese monkey showed no cross reaction at all. Cross reaction was noted with a chimpanzee, but experimentally it could be differentiated from human. Furthermore, no cross reaction was noted with respect to blood samples from non-primates (guinea pig, sheep, horse, dog and cat).
Authors:
Y Yamamoto; A Tsutsumi; H Ishizu
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Forensic science international     Volume:  40     ISSN:  0379-0738     ISO Abbreviation:  Forensic Sci. Int.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-04-21     Completed Date:  1989-04-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7902034     Medline TA:  Forensic Sci Int     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  85-95     Citation Subset:  IM    
Affiliation:
Department of Legal Medicine, Kochi Medical School, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / diagnostic use*
Antibody Specificity
Blood Stains / legislation & jurisprudence*
Enzyme-Linked Immunosorbent Assay*
Humans
Immunoglobulin Light Chains / analysis*
Immunoglobulin kappa-Chains / analysis*
Species Specificity*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Immunoglobulin Light Chains; 0/Immunoglobulin kappa-Chains

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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