Document Detail

Species-Specific Activity of Glycolipid Ligands for Invariant NKT Cells.
MedLine Citation:
PMID:  22639457     Owner:  NLM     Status:  Publisher    
The immunomodulatory glycolipid α-galactosylceramide (α-GalCer) binds to CD1d and exhibits potent activity as a ligand for invariant CD1d-restricted natural killer-like T cells (iNKT cells). Structural analogues of α-GalCer have been synthesised to determine which components are required for CD1d presentation and iNKT cell activation, however, to date the importance of the phytosphingosine 4-hydroxyl for iNKT cell activation has been disputed. To clarify this, we synthesised two 4-deoxy α-GalCer analogues (sphinganine and sphingosine) and investigated their ability to activate murine and human iNKT cells. Analysis revealed that the analogues possessed comparable activity to α-GalCer in stimulating murine iNKT cells, but were severely compromised in their ability to stimulate human iNKT cells. Here we determined that species-specific glycolipid activity was due to a lack of recognition of the analogues by the T-cell receptors on human iNKT cells rather than insufficient presentation of the analogues on human CD1d molecules. From these results we suggest that glycolipids developed for potent iNKT cell activity in humans should contain a phytosphingosine base.
Emma M Dangerfield; Janice M H Cheng; Deborah A Knight; Robert Weinkove; P Rod Dunbar; Ian F Hermans; Mattie S M Timmer; Bridget L Stocker
Related Documents :
476147 - Partition behaviour of a cultured mouse mammary cancer cell line in aqueous two-phase p...
2913827 - Cell cycle perturbation and cell death after exposure of a human lymphoblastoid cell st...
1819537 - The effect of herbicide "chwastox płynny 30" on the cell cycle parameters in root meri...
3866897 - Comparison of autoradiography and dna histograms in assessing s-phase cells from patien...
8876147 - A stable human-derived packaging cell line for production of high titer retrovirus/vesi...
9349607 - In utero undernutrition impairs rat beta-cell development.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-25
Journal Detail:
Title:  Chembiochem : a European journal of chemical biology     Volume:  -     ISSN:  1439-7633     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100937360     Medline TA:  Chembiochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Malaghan Institute of Medical Research, P. O. Box 7060, Wellington 6242 (New Zealand); School of Chemical and Physical Sciences and, School of Biological Sciences, Victoria University of Wellington, P. O. Box 600, Wellington 6242 (New Zealand).
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Elements for a Rational Polymer Approach towards Carbon Nanostructures.
Next Document:  Balloon assisted tracking of a guide catheter through difficult radial anatomy: A technical report.