Document Detail


Speciation of Inorganic- and Methyl-Mercury in Biological Matrices by Electrochemical Vapor Generation from an L-cysteine modified graphite electrode with Atomic Fluorescence Spectrometry detection.
MedLine Citation:
PMID:  23035833     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A novel non-chromatographic speciation technique for ultratrace inorganic mercury (Hg2+) and methylmercury (CH3Hg+) in biological materials is developed and validated by electrolytic vapor generation (EVG) coupled with atomic fluorescence spectrometry (AFS). The studies show that CH3Hg+ and Hg2+ can be conversed to Hg vapor efficiently on an L-cysteine modified graphite cathode, which has never been reported before. We observe that only Hg2+ can be conversed efficiently to Hg vapor at low current mode (0.2 A). While at high current mode (2.2 A), both CH3Hg+ and Hg2+ can be reduced efficiently. As a result, we successfully establish an exact and sensitive method based on the current control to detect mercury speciation for the first time. The factors of electrolytic conditions have been optimized and the potential mechanism is discussed. Under the optimal conditions, the detection limits (3s) of Hg2+ and CH3Hg+ in aqueous solutions are 0.098 μg L-1and 0.073 μg L-1, respectively. The relative standard deviations for 6 replicate determinations of 2 μg L-1 Hg are determined as 3.2% and 4.7% for Hg2+ and CH3Hg+. The accuracy of the method is verified through the analysis of certified reference materials (CRM, NRC-DORM-2) and the proposed method has been applied satisfactorily to the determination of mercury speciation in several seafood samples by calibration curve mode.
Authors:
Wangbing Zhang; Xin-An Yang; Yong-Ping Dong; Jing-Jing Xue
Related Documents :
23625703 - Validation of a novel immunoassay for the detection of synthetic cannabinoids and metab...
23244593 - Activity-based exposure comparisons among humans and nonclinical safety testing species...
20712133 - Comparison of liquid chromatography-ultraviolet and chromatography-tandem mass spectrom...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effects of mechanical properties of thermoplastic materials on the initial force of thermoplastic ap...
Next Document:  Feasibility of a nursing rehabilitation guideline for patients with stroke: evaluating the use by nu...