| Spatiotemporal progression of cell death in the zone of ischemia surrounding burns. | |
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MedLine Citation:
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PMID: 22092800 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Burns are dynamic injuries, characterized by progressive death of surrounding tissue over time. Although central to an understanding of burn injury progression, the spatiotemporal degrees and rates of cellular necrosis and apoptosis in the zone of ischemia surrounding burns are not well characterized. Using a validated porcine hot comb model, we probed periburn tissue at 1, 4, and 24 hours after injury for high-mobility group box 1 as a marker of necrosis and activated cleaved caspase-3 as a marker of apoptosis, followed by spatiotemporal morphometric analysis. We found that necrosis was the most prominent mechanism of cell death in burn injury progression, with significant progression between 1 and 4 hours postburn. Apoptosis appeared not to play a role in early burn injury progression but was observed in cells at the interface of necrotic and viable tissue at 24 hours postburn. Our findings imply that intervention within the first 4 hours following injury is likely necessary to limit burn injury progression. Additionally, based on high-mobility group box 1 staining patterns, we define distinct early, intermediate, and late pathological signs of cell necrosis that may facilitate delineation of causal mechanistic relationships of burn injury progression in vivo. |
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Authors:
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Steven T Lanier; Steve A McClain; Fubao Lin; Adam J Singer; Richard A F Clark |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society Volume: 19 ISSN: 1524-475X ISO Abbreviation: Wound Repair Regen Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-11-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9310939 Medline TA: Wound Repair Regen Country: United States |
Other Details:
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Languages: eng Pagination: 622-32 Citation Subset: IM |
Copyright Information:
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© 2011 by the Wound Healing Society. |
Affiliation:
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Stony Brook University School of Medicine, Stony Brook, New York. |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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