Document Detail


Spatiotemporal patterns of activity in an intact mammalian network with single-cell resolution: optical studies of nicotinic activity in an enteric plexus.
MedLine Citation:
PMID:  10191324     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Multiple Site Optical Recording of Transmembrane Voltage (MSORTV) has been used to measure, continuously and simultaneously, the spontaneous electrical activity from all of the neurons in individual ganglia or up to five interconnected ganglia of the submucous plexus of the guinea pig small intestine. These are the first optical recordings of electrical activity with single-cell resolution from a mammalian nervous system. They are used to investigate the effects of acute and chronic application of nicotine on the firing patterns of this neural network containing important cholinergic components. After washout of acutely applied nicotine, the firing rates of selected neurons were dramatically elevated. These results suggest that nAChRs that reversibly desensitize after exposure to nicotine may be responsible for the enhancement of activity that is observed after a brief application of this agonist. In addition, immunostaining with monoclonal antibodies was used to localize alpha3/alpha5, alpha7, and beta2 nAChR subunits, and the results demonstrate the prevalence of alpha3/alpha5. It is this alpha3-containing nAChR subtype that probably accounts for most of the excess activity elicited by nicotine application.
Authors:
A L Obaid; T Koyano; J Lindstrom; T Sakai; B M Salzberg
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  19     ISSN:  0270-6474     ISO Abbreviation:  J. Neurosci.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-04-27     Completed Date:  1999-04-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3073-93     Citation Subset:  IM    
Affiliation:
Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6074, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Guinea Pigs
Immunohistochemistry
Membrane Potentials / physiology
Nerve Net / physiology*
Neurons / physiology*
Optics and Photonics*
Receptors, Nicotinic / analysis,  physiology*
Submucous Plexus / physiology*
Grant Support
ID/Acronym/Agency:
NS11323/NS/NINDS NIH HHS; NS16824/NS/NINDS NIH HHS; NS35561/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Nicotinic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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