Document Detail

Spatiotemporal coordinated hierarchical properties of cellular protrusion revealed by multiscale analysis.
MedLine Citation:
PMID:  22689105     Owner:  NLM     Status:  Publisher    
We present a methodology for integrative multiscale analysis to highlight hierarchical properties of cellular protrusion and mechanochemical interactions in cellular protrusion based on live cell imaging data with high spatiotemporal resolution. As an appropriate experimental system, we selected non-polarized full-moon-shaped keratocytes that present balanced protrusion around the entire cell periphery at the cellular scale simultaneously with active protrusion and retraction at the subcellular scale. We achieved the observation of a whole cell with sub-micrometer spatial precision and sub-second time resolution for three minutes or more. The multiscale characteristics of cell peripheral activity and those of the cell peripheral shape were extracted from an identical image sequence by estimating the cell protrusion rates and the cell peripheral curvatures at various differential intervals. The spatiotemporal maps of the cell protrusion rates demonstrated a spatiotemporally nested structure of travelling waves of active protruding regions at the cellular and subcellular scales. Moreover, correlation analysis demonstrated the relationship between the cell protrusion rate and peripheral curvature at the subcellular scale. The novel integrative methodology presented here well highlighted the hierarchical properties of organized cellular protrusion, and further provided insight about the underlying mechanochemical interactions between the cell membrane and the actin filaments under the membrane.
Hiromi Miyoshi; Taiji Adachi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-11
Journal Detail:
Title:  Integrative biology : quantitative biosciences from nano to macro     Volume:  -     ISSN:  1757-9708     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101478378     Medline TA:  Integr Biol (Camb)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Ultrahigh Precision Fabrication Team, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
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