Document Detail


Spatiotemporal coordinated hierarchical properties of cellular protrusion revealed by multiscale analysis.
MedLine Citation:
PMID:  22689105     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We present a methodology for integrative multiscale analysis to highlight hierarchical properties of cellular protrusion and mechanochemical interactions in cellular protrusion based on live cell imaging data with high spatiotemporal resolution. As an appropriate experimental system, we selected non-polarized full-moon-shaped keratocytes that present balanced protrusion around the entire cell periphery at the cellular scale simultaneously with active protrusion and retraction at the subcellular scale. We achieved the observation of a whole cell with sub-micrometer spatial precision and sub-second time resolution for three minutes or more. The multiscale characteristics of cell peripheral activity and those of the cell peripheral shape were extracted from an identical image sequence by estimating the cell protrusion rates and the cell peripheral curvatures at various differential intervals. The spatiotemporal maps of the cell protrusion rates demonstrated a spatiotemporally nested structure of travelling waves of active protruding regions at the cellular and subcellular scales. Moreover, correlation analysis demonstrated the relationship between the cell protrusion rate and peripheral curvature at the subcellular scale. The novel integrative methodology presented here well highlighted the hierarchical properties of organized cellular protrusion, and further provided insight about the underlying mechanochemical interactions between the cell membrane and the actin filaments under the membrane.
Authors:
Hiromi Miyoshi; Taiji Adachi
Related Documents :
15518925 - Use of fluorescence imaging to investigate the structure and function of intestinal m c...
19381815 - High-throughput microfluidic system for monitoring diffusion-based monolayer yeast cell...
18312825 - Isostructural fluorescent and radioactive probes for monitoring neural stem and progeni...
438505 - Polarization of fluorescein fluorescence in single cells.
23724055 - Electrofusion of mesenchymal stem cells and islet cells for diabetes therapy: a rat model.
8625745 - Inhibition of myofibroblastic transformation of cultured rat hepatic stellate cells by ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-11
Journal Detail:
Title:  Integrative biology : quantitative biosciences from nano to macro     Volume:  -     ISSN:  1757-9708     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101478378     Medline TA:  Integr Biol (Camb)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Ultrahigh Precision Fabrication Team, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan. hiromi-miyoshi@riken.jp.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  No Effect of the IGF-1 Gene rs35767 and rs17032362 Polymorphisms in the Etiology of Idiopathic Short...
Next Document:  [A screening program for colorectal cancer in Chilean subjects aged fifty years or more].