| Spatiometabolic stratification of Shewanella oneidensis biofilms. | |
MedLine Citation:
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PMID: 16936048 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Biofilms, or surface-attached microbial communities, are both ubiquitous and resilient in the environment. Although much is known about how biofilms form, develop, and detach, very little is understood about how these events are related to metabolism and its dynamics. It is commonly thought that large subpopulations of cells within biofilms are not actively producing proteins or generating energy and are therefore dead. An alternative hypothesis is that within the growth-inactive domains of biofilms, significant populations of living cells persist and retain the capacity to dynamically regulate their metabolism. To test this, we employed unstable fluorescent reporters to measure growth activity and protein synthesis in vivo over the course of biofilm development and created a quantitative routine to compare domains of activity in independently grown biofilms. Here we report that Shewanella oneidensis biofilm structures reproducibly stratify with respect to growth activity and metabolism as a function of size. Within domains of growth-inactive cells, genes typically upregulated under anaerobic conditions are expressed well after growth has ceased. These findings reveal that, far from being dead, the majority of cells in mature S. oneidensis biofilms have actively turned-on metabolic programs appropriate to their local microenvironment and developmental stage. |
Authors:
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Tracy K Teal; Douglas P Lies; Barbara J Wold; Dianne K Newman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2006-08-25 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 72 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-11-07 Completed Date: 2007-01-23 Revised Date: 2013-06-07 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 7324-30 Citation Subset: IM |
Affiliation:
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Division of Biological Sciences, California Institute of Technology, Pasadena, California 91125, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anaerobiosis Bacterial Outer Membrane Proteins / genetics, metabolism Bacterial Proteins / genetics, metabolism* Biofilms / growth & development* Culture Media Gene Expression Regulation, Bacterial* Green Fluorescent Proteins / genetics, metabolism Organic Chemicals / metabolism Plasmids Shewanella / growth & development*, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/Culture Media; 0/MtrB protein, Shewanella putrefaciens; 0/Organic Chemicals; 0/SYTO 9; 147336-22-9/Green Fluorescent Proteins |
| Comments/Corrections | |
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