Document Detail

Spatially directed guidance of stem cell population migration by immobilized patterns of growth factors.
MedLine Citation:
PMID:  21272933     Owner:  NLM     Status:  MEDLINE    
We investigated how engineered gradients of exogenous growth factors, immobilized to an extracellular matrix material, influence collective guidance of stem cell populations over extended time (>1 day) and length (>1 mm) scales in vitro. Patterns of low-to-high, high-to-low, and uniform concentrations of heparin-binding epidermal growth factor-like growth factor were inkjet printed at precise locations on fibrin substrates. Proliferation and migration responses of mesenchymal stem cells seeded at pattern origins were observed with time-lapse video microscopy and analyzed using both manual and automated computer vision-based cell tracking techniques. Based on results of established chemotaxis studies, we expected that the low-to-high gradient would most effectively direct cell guidance away from the cell source. All printed patterns, however, were found to direct net collective cell guidance with comparable responses. Our analysis revealed that collective "cell diffusion" down a cell-to-cell confinement gradient originating at the cell starting lines and not the net sum of directed individual cell migration up a growth factor concentration gradient is the principal driving force for directing mesenchymal stem cell population outgrowth from a cell source. These results suggest that simple uniform distributions of growth factors immobilized to an extracellular matrix material may be as effective in directing cell migration into a wound site as more complex patterns with concentration gradients.
Eric D Miller; Kang Li; Takeo Kanade; Lee E Weiss; Lynn M Walker; Phil G Campbell
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2011-01-26
Journal Detail:
Title:  Biomaterials     Volume:  32     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-02-21     Completed Date:  2011-06-01     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  2775-85     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
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MeSH Terms
Biocompatible Materials / chemistry
Cell Line
Cell Movement / drug effects
Cell Proliferation / drug effects
Extracellular Matrix / chemistry
Intercellular Signaling Peptides and Proteins / chemistry,  pharmacology*
Mesenchymal Stromal Cells / cytology*,  drug effects*
Grant Support
R01 EB004343/EB/NIBIB NIH HHS; R01 EB004343/EB/NIBIB NIH HHS; R01 EB004343-03/EB/NIBIB NIH HHS; R01 EB004343-04/EB/NIBIB NIH HHS; R01 EB007369/EB/NIBIB NIH HHS; R01 EB007369/EB/NIBIB NIH HHS; R01 EB007369-03/EB/NIBIB NIH HHS; R01 EB007369-04/EB/NIBIB NIH HHS
Reg. No./Substance:
0/Biocompatible Materials; 0/Intercellular Signaling Peptides and Proteins; 149176-25-0/heparin-binding EGF-like growth factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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