Document Detail


Spatial and temporal properties of ventral blood island induction in Xenopus laevis.
MedLine Citation:
PMID:  10556058     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Questions of dorsoventral axis determination and patterning in Xenopus seek to uncover the mechanisms by which particular mesodermal fates, for example somite, are specified in the dorsal pole of the axis while other mesoderm fates, for example, ventral blood island (VBI), are specified at the ventral pole. We report here that the genes Xvent-1, Xvent-2, and Xwnt-8 do not appear to be in the pathway of VBI induction, contrary to previous reports. Results from the selective inhibition of bone morphogenetic protein (BMP) activity, a key regulator of VBI induction, by ectopic Noggin, Chordin, or dominant negative BMP ligands and receptors suggest an alternative route of VBI induction. Injection of noggin or chordin RNA into animal pole blastomeres effectively inhibited VBI development, while marginal zone injection had no effect. Cell autonomous inhibition of BMP activity in epidermis with dominant negative ligand dramatically reduced the amount of (&agr;)T3 globin expression. These results indicate that signaling activity from the Spemann Organizer alone may not be sufficient for dorsoventral patterning in the marginal zone and that an inductive interaction between presumptive VBIs and ectoderm late in gastrulation may be crucial. In agreement with these observations, other results show that in explanted blastula-stage marginal zones a distinct pattern develops with a restricted VBI-forming region at the vegetal pole that is independent of the patterning activity of the Spemann Organizer.
Authors:
G Kumano; L Belluzzi; W C Smith
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  126     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-01-06     Completed Date:  2000-01-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  5327-37     Citation Subset:  IM    
Affiliation:
Department of Molecular, Cellular and Developmental Biology, and Neuroscience Research Institute, University of California, Santa Barbara, CA 93106, USA.
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MeSH Terms
Descriptor/Qualifier:
Actins / genetics,  metabolism
Animals
Blastomeres
Body Patterning / genetics
Bone Morphogenetic Protein 4
Bone Morphogenetic Protein 7
Bone Morphogenetic Proteins / genetics,  metabolism
Carrier Proteins
Embryo, Nonmammalian
Embryonic Induction / genetics
Female
Gastrula
Gene Expression Regulation, Developmental*
Globins / genetics,  metabolism
Glycoproteins*
Homeodomain Proteins / genetics,  metabolism
Intercellular Signaling Peptides and Proteins*
Mesoderm / transplantation
Morphogenesis
Mutation
MyoD Protein / genetics,  metabolism
Organizers, Embryonic / physiology
Proteins / genetics,  metabolism
Proto-Oncogene Proteins / genetics,  metabolism
Signal Transduction
Transcription Factors*
Transforming Growth Factor beta*
Wnt Proteins
Xenopus Proteins*
Xenopus laevis / blood*,  embryology*
Zebrafish Proteins*
Grant Support
ID/Acronym/Agency:
GM52835/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/BMP7 protein, zebrafish; 0/Bone Morphogenetic Protein 4; 0/Bone Morphogenetic Protein 7; 0/Bone Morphogenetic Proteins; 0/Carrier Proteins; 0/Glycoproteins; 0/Homeodomain Proteins; 0/Intercellular Signaling Peptides and Proteins; 0/MyoD Protein; 0/Proteins; 0/Proto-Oncogene Proteins; 0/Transcription Factors; 0/Transforming Growth Factor beta; 0/Wnt Proteins; 0/Xenopus Proteins; 0/Xvent-1 protein, Xenopus; 0/Xvent-2 protein, Xenopus; 0/Zebrafish Proteins; 0/bmp4 protein, Xenopus; 0/bmp4 protein, zebrafish; 0/bmp7.1 protein, Xenopus; 0/wnt8a protein, Xenopus; 148294-77-3/noggin protein; 9004-22-2/Globins; 93586-27-7/chordin

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