Document Detail

Spatial-temporal dynamics of collective chemosensing.
MedLine Citation:
PMID:  22566661     Owner:  NLM     Status:  MEDLINE    
Although the process of chemosensing by individual cells is intrisically stochastic, multicellular organisms exhibit highly regulated responses to external stimulations. Two key elements to understand the deterministic features of chemosensing are intercellular communications and the role of pacemaker cells. To characterize the collective behavior induced by these two factors, we study the spatial-temporal calcium dynamics of fibroblast cells in response to ATP stimulation. We find that closely packed cell colonies exhibit faster, more synchronized, and highly correlated responses compared to isolated cells. In addition, we demonstrate for chemosensing the existence of pacemaker cells and how the presence of gap junctions impact the first step of the collective response. By further comparing these results with the calcium dynamics of cells embedded in thin hydrogel films, where intercellular communication is only possible via diffusing molecules, we conclude that gap junctions are required for synchronized and highly correlated responses among cells in high density colonies. In addition, in high density cell colonies, both communication channels lead to calcium oscillations following the stimulation by external ATP. While the calcium oscillations associated with cells directly exposed to external flows were transient, the oscillations of hydrogel trapped cells can persist with a fundamental frequency and higher harmonics. Our observations and measurements highlight the crucial role of intercellular signaling for generating regulated spatial and temporal dynamics in cell colonies and tissues.
Bo Sun; Josephine Lembong; Valery Normand; Matthew Rogers; Howard A Stone
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-07
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  109     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-16     Completed Date:  2012-07-27     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7753-8     Citation Subset:  IM    
Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, USA.
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MeSH Terms
Adenosine Triphosphate / metabolism*
Biological Clocks / physiology
Calcium / metabolism*
Cell Communication / physiology*
Cell Count
Fibroblasts / metabolism*
Gap Junctions / physiology*
Image Processing, Computer-Assisted
Microscopy, Fluorescence
NIH 3T3 Cells
Reg. No./Substance:
25852-47-5/Hydrogel; 56-65-5/Adenosine Triphosphate; 7440-70-2/Calcium
Comment In:
Proc Natl Acad Sci U S A. 2012 May 15;109(20):7591-2   [PMID:  22570502 ]

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