| Spatial-temporal dynamics of collective chemosensing. | |
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MedLine Citation:
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PMID: 22566661 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although the process of chemosensing by individual cells is intrisically stochastic, multicellular organisms exhibit highly regulated responses to external stimulations. Two key elements to understand the deterministic features of chemosensing are intercellular communications and the role of pacemaker cells. To characterize the collective behavior induced by these two factors, we study the spatial-temporal calcium dynamics of fibroblast cells in response to ATP stimulation. We find that closely packed cell colonies exhibit faster, more synchronized, and highly correlated responses compared to isolated cells. In addition, we demonstrate for chemosensing the existence of pacemaker cells and how the presence of gap junctions impact the first step of the collective response. By further comparing these results with the calcium dynamics of cells embedded in thin hydrogel films, where intercellular communication is only possible via diffusing molecules, we conclude that gap junctions are required for synchronized and highly correlated responses among cells in high density colonies. In addition, in high density cell colonies, both communication channels lead to calcium oscillations following the stimulation by external ATP. While the calcium oscillations associated with cells directly exposed to external flows were transient, the oscillations of hydrogel trapped cells can persist with a fundamental frequency and higher harmonics. Our observations and measurements highlight the crucial role of intercellular signaling for generating regulated spatial and temporal dynamics in cell colonies and tissues. |
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Authors:
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Bo Sun; Josephine Lembong; Valery Normand; Matthew Rogers; Howard A Stone |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-05-07 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 109 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-05-16 Completed Date: 2012-07-27 Revised Date: 2013-02-19 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 7753-8 Citation Subset: IM |
Affiliation:
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Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism* Animals Biological Clocks / physiology Calcium / metabolism* Cell Communication / physiology* Cell Count Fibroblasts / metabolism* Gap Junctions / physiology* Hydrogel Image Processing, Computer-Assisted Mice Microscopy, Fluorescence NIH 3T3 Cells |
| Chemical | |
Reg. No./Substance:
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25852-47-5/Hydrogel; 56-65-5/Adenosine Triphosphate; 7440-70-2/Calcium |
| Comments/Corrections | |
Comment In:
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Proc Natl Acad Sci U S A. 2012 May 15;109(20):7591-2
[PMID:
22570502
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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