Document Detail

Spatial-temporal distribution of nitric oxide involved in regulation of phenylalanine ammonialyase activation and Taxol production in immobilized Taxus cuspidata cells.
MedLine Citation:
PMID:  19103236     Owner:  NLM     Status:  MEDLINE    
The generation of nitric oxide (NO) in Taxus cuspidata in immobilized support matrices and the potential role of NO as signal molecular in regulation of Taxol production were investigated. It was found that the immobilization induced a spatial and temporal-dependent NO burst in immobilized supported matrices. NO level reached the maximum in the central zone of immobilized supported matrices on day 20, which was more than twice compared with that in suspended cells. Further investigations showed that the phenylalanine ammonialyase (PAL) activity and Taxol production of the 20-day-old immobilized T. cuspidata cells increased by onefold and 11% after 4h treatment with 20 microM NO donor (sodium nitroprusside), respectively. NO inhibitor N(omega)-nitro-L-arginine and NO scavenger 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxyde partially blocked PAL activity and Taxol accumulation in immobilized cells. These results suggest that NO plays a signal role in regulation of PAL activity and Taxol production in immobilized T. cuspidata cells.
Wen-Hai Xiao; Jing-sheng Cheng; Ying-Jin Yuan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-03
Journal Detail:
Title:  Journal of biotechnology     Volume:  139     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-03     Completed Date:  2009-04-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  222-8     Citation Subset:  IM    
Department of Pharmaceutical Engineering, Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China.
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MeSH Terms
Analysis of Variance
Cells, Immobilized / cytology,  metabolism*
Free Radical Scavengers / pharmacology
Nitric Oxide / metabolism*
Nitric Oxide Donors / pharmacology
Nitric Oxide Synthase / antagonists & inhibitors,  pharmacology
Paclitaxel / metabolism*
Phenylalanine Ammonia-Lyase / metabolism*
Signal Transduction / drug effects,  physiology
Taxus / cytology,  metabolism*
Reg. No./Substance:
0/Free Radical Scavengers; 0/Nitric Oxide Donors; 10102-43-9/Nitric Oxide; 33069-62-4/Paclitaxel; EC Oxide Synthase; EC Ammonia-Lyase

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