Document Detail


Spatial regulation of specific gene expression through photoactivation of RNAi.
MedLine Citation:
PMID:  19376174     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.
Authors:
Tamaki Endoh; Masahiko Sisido; Takashi Ohtsuki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-17
Journal Detail:
Title:  Journal of controlled release : official journal of the Controlled Release Society     Volume:  137     ISSN:  1873-4995     ISO Abbreviation:  J Control Release     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-06-29     Completed Date:  2009-10-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8607908     Medline TA:  J Control Release     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  241-5     Citation Subset:  IM    
Affiliation:
Department of Bioscience and Biotechnology, Okayama University, Okayama 700-8530, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Cricetinae
Cricetulus
Drug Carriers / administration & dosage,  chemistry
Fluorescent Dyes / administration & dosage,  chemistry*
Gene Products, tat / administration & dosage,  chemistry*
Lasers
Photochemistry*
RNA Interference*
RNA, Small Interfering / administration & dosage*
Recombinant Fusion Proteins / administration & dosage,  chemistry
Ribonucleoprotein, U1 Small Nuclear / administration & dosage,  chemistry*
Chemical
Reg. No./Substance:
0/Drug Carriers; 0/Fluorescent Dyes; 0/Gene Products, tat; 0/RNA, Small Interfering; 0/Recombinant Fusion Proteins; 0/Ribonucleoprotein, U1 Small Nuclear; 0/U1A protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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