Document Detail


Spatial regulation of Dia and Myosin-II by RhoGEF2 controls initiation of E-cadherin endocytosis during epithelial morphogenesis.
MedLine Citation:
PMID:  21516109     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
E-cadherin plays a pivotal role in epithelial morphogenesis. It controls the intercellular adhesion required for tissue cohesion and anchors the actomyosin-driven tension needed to change cell shape. In the early Drosophila embryo, Myosin-II (Myo-II) controls the planar polarized remodelling of cell junctions and tissue extension. The E-cadherin distribution is also planar polarized and complementary to the Myosin-II distribution. Here we show that E-cadherin polarity is controlled by the polarized regulation of clathrin- and dynamin-mediated endocytosis. Blocking E-cadherin endocytosis resulted in cell intercalation defects. We delineate a pathway that controls the initiation of E-cadherin endocytosis through the regulation of AP2 and clathrin coat recruitment by E-cadherin. This requires the concerted action of the formin Diaphanous (Dia) and Myosin-II. Their activity is controlled by the guanine exchange factor RhoGEF2, which is planar polarized and absent in non-intercalating regions. Finally, we provide evidence that Dia and Myo-II control the initiation of E-cadherin endocytosis by regulating the lateral clustering of E-cadherin.
Authors:
Romain Levayer; Anne Pelissier-Monier; Thomas Lecuit
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-04-24
Journal Detail:
Title:  Nature cell biology     Volume:  13     ISSN:  1476-4679     ISO Abbreviation:  Nat. Cell Biol.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-04     Completed Date:  2011-07-12     Revised Date:  2011-07-22    
Medline Journal Info:
Nlm Unique ID:  100890575     Medline TA:  Nat Cell Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  529-40     Citation Subset:  IM    
Affiliation:
IBDML, UMR6216 CNRS-Université de la Méditerranée, Campus de Luminy, case 907. 13288 Marseille Cedex 09, France.
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MeSH Terms
Descriptor/Qualifier:
Adherens Junctions
Animals
Cadherins / metabolism*
Cell Shape
Clathrin / metabolism
Drosophila / embryology
Drosophila Proteins / physiology*
Embryo, Nonmammalian / cytology
Endocytosis*
Epithelial Cells / cytology*
Lymphokines / physiology*
Morphogenesis
Myosin Type II / physiology*
rho GTP-Binding Proteins / physiology*
Chemical
Reg. No./Substance:
0/Cadherins; 0/Clathrin; 0/Drosophila Proteins; 0/Lymphokines; 0/monocyte-macrophage differentiation factor; EC 3.6.1.-/Myosin Type II; EC 3.6.1.-/shar-pei protein, Drosophila; EC 3.6.5.2/rho GTP-Binding Proteins
Comments/Corrections
Erratum In:
Nat Cell Biol. 2011 Jun;13(6):734

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