Document Detail


Spatial organization of lipids in the human retina and optic nerve by MALDI imaging mass spectrometry.
MedLine Citation:
PMID:  24367044     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) was used to characterize lipid species within sections of human eyes. Common phospholipids, such as PC(34:1), abundant in most tissues, were observed in accessory tissue, optic nerve, and retina. Triacylglycerols were highly localized in accessory tissue, whereas sulfatide and plasmalogen gylcerophosphoethanolamine (PE) lipids with a monounsaturated fatty acid were found enriched in optic nerves. Additionally, several lipids were associated solely with inner retina, photoreceptors, or retinal pigment epithelium; a plasmalogen PE lipid containing docosahexaenoic acid (DHA), PE(18:0p/22:6), was present exclusively in inner retina, and glycerophosphocholine (PC) and PE diacyl DHA-containing lipids were found solely in photoreceptors. PC lipids containing very long chain polyunsaturated fatty acids (VLC-PUFA) were detected in photoreceptors despite their low abundance in retina. Ceramide lipids and the bis-retinoid, A2E, were present only in retinal pigment epithelium. This MALDI IMS study readily revealed the location of many lipids that have been associated with degenerative retinal diseases. Complex lipid localization within retinal tissue provides a global view of lipid organization and initial evidence for specific functions in localized regions, offering opportunities to assess their significance in retinal diseases, such as macular degeneration, where lipids have been implicated in the disease process.
Authors:
Karin A Zemski Berry; William C Gordon; Robert C Murphy; Nicolas G Bazan
Related Documents :
8464794 - Evaluation of egg injection of folic acid and effect of supplemental folic acid on hatc...
18371764 - Determination of folic acid by chemiluminescence based on peroxomonosulfate-cobalt(ii) ...
8972234 - Evaluation of the developmental toxicity of theophylline, dimethyluric acid, and methyl...
11390184 - Free radical scavenging behavior of folic acid: evidence for possible antioxidant activ...
16996264 - Feasibility of methanogenic digestion applied to a low ph acetic acid solution.
11199364 - Intragastric co2 and nitric oxide participate in the regulation of peptone-induced gast...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-12-23
Journal Detail:
Title:  Journal of lipid research     Volume:  -     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2013 Dec 
Date Detail:
Created Date:  2013-12-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Everolimus Dramatically Improves Glycemic Control in Unresectable Metastatic Insulinoma: A Case Repo...
Next Document:  Novel approach in LC-MS/MS using MRM to generate a full profile of acyl-CoAs; discovery of acyl-deph...