| Spatial localization of bacteria controls coagulation of human blood by 'quorum acting'. | |
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MedLine Citation:
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PMID: 19031531 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Blood coagulation often accompanies bacterial infections and sepsis and is generally accepted as a consequence of immune responses. Though many bacterial species can directly activate individual coagulation factors, they have not been shown to directly initiate the coagulation cascade that precedes clot formation. Here we demonstrated, using microfluidics and surface patterning, that the spatial localization of bacteria substantially affects coagulation of human and mouse blood and plasma. Bacillus cereus and Bacillus anthracis, the anthrax-causing pathogen, directly initiated coagulation of blood in minutes when bacterial cells were clustered. Coagulation of human blood by B. anthracis required secreted zinc metalloprotease InhA1, which activated prothrombin and factor X directly (not via factor XII or tissue factor pathways). We refer to this mechanism as 'quorum acting' to distinguish it from quorum sensing--it does not require a change in gene expression, it can be rapid and it can be independent of bacterium-to-bacterium communication. |
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Authors:
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Christian J Kastrup; James Q Boedicker; Andrei P Pomerantsev; Mahtab Moayeri; Yao Bian; Rebecca R Pompano; Timothy R Kline; Patricia Sylvestre; Feng Shen; Stephen H Leppla; Wei-Jen Tang; Rustem F Ismagilov |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Nature chemical biology Volume: 4 ISSN: 1552-4469 ISO Abbreviation: Nat. Chem. Biol. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-18 Completed Date: 2008-12-24 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 101231976 Medline TA: Nat Chem Biol Country: United States |
Other Details:
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Languages: eng Pagination: 742-50 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacillus anthracis Bacillus cereus Bacterial Infections / complications* Bacterial Physiological Phenomena* Blood Coagulation* Factor X Humans Metalloproteases Mice Microfluidics Models, Biological Prothrombin Quorum Sensing |
| Grant Support | |
ID/Acronym/Agency:
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DP1 OD003584-01/OD/NIH HHS; DP1OD003584/OD/NIH HHS; GM 62548/GM/NIGMS NIH HHS; GM 81539/GM/NIGMS NIH HHS; R01 GM062548-08/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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9001-26-7/Prothrombin; 9001-29-0/Factor X; EC 3.4.-/Metalloproteases |
| Comments/Corrections | |
Comment In:
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Nat Chem Biol. 2008 Dec;4(12):718-9
[PMID:
19008879
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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