Document Detail


Spatial distribution and initial changes of SSEA-1 and other cell adhesion-related molecules on mouse embryonic stem cells before and during differentiation.
MedLine Citation:
PMID:  15505339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We examined the distribution of cell adhesion-related molecules (CAMs) among mouse embryonic stem (ES) cells and the spatial distribution on cell surfaces before and during differentiation. The cell-cell heterogeneity of SSEA-1, PECAM-1, and ICAM-1 among the undifferentiated cells in the ES cell colonies was evident by immunohistochemistry and immuno-SEM, supporting the flow cytometry findings. In contrast, most undifferentiated ES cells strongly expressed CD9. SSEA-1 was located preferentially on the edge of low protuberances and microvilli and formed clusters or linear arrays of 3-20 particles. PECAM-1 and ICAM-1 were randomly localized on the free cell surfaces, whereas CD9 was preferentially localized on the microvilli or protuberances, especially in the cell periphery. Both the SSEA-1(+) fraction and the SSEA-1(-) fraction of magnetic cell sorting (MACS) formed undifferentiated colonies after plating. Flow cytometry showed that these populations reverted separately again to a culture with a mixed phenotype. Differentiation induced by retinoic acid downregulated the expression of all CAMs. Immuno-SEM showed decreases of SSEA-1 in the differentiated ES cells, although some clustering still remained. Our findings help to elucidate the significance of these molecules in ES cell maintenance and differentiation and suggest that cell surface antigens may be useful for defining the phenotype of undifferentiated and differentiated ES cells.
Authors:
Li Cui; Kohei Johkura; Fengming Yue; Naoko Ogiwara; Yasumitsu Okouchi; Kazuhiko Asanuma; Katsunori Sasaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  52     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-10-26     Completed Date:  2004-12-08     Revised Date:  2014-06-08    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1447-57     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD / biosynthesis*
Antigens, CD15 / biosynthesis*
Antigens, CD31 / biosynthesis*
Antigens, CD9
Cell Adhesion
Cell Differentiation
Cells, Cultured
Embryo, Mammalian / cytology
Flow Cytometry
Intercellular Adhesion Molecule-1 / biosynthesis*
Membrane Glycoproteins / biosynthesis*
Mice
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells / cytology,  metabolism*,  ultrastructure
Tretinoin / pharmacology
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD15; 0/Antigens, CD31; 0/Antigens, CD9; 0/Cd9 protein, mouse; 0/Membrane Glycoproteins; 126547-89-5/Intercellular Adhesion Molecule-1; 5688UTC01R/Tretinoin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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