Document Detail

Spatial confinement of neurite regrowth from dorsal root ganglia within nonporous microconduits.
MedLine Citation:
PMID:  12740083     Owner:  NLM     Status:  MEDLINE    
Tissue engineering is founded on the concept of controlling the behavior of individual cells to stimulate tissue formation. This control is achieved by mimicking signals that manage natural tissue development or repair. These interdependent signals include cytokine delivery, extracellular matrix interactions, and cell-cell communication. Here, we report on the effect of spatial guidance as a signal for nerve tissue regeneration, using a simple in vitro model. We observe the acceleration of neurite extension from rat dorsal root ganglia within micron-scale tubes. Within these hydrogel-filled conduits, neurites were observed to extend more rapidly than when cultured within the hydrogel alone. The spatial cue also induced a change in tissue architecture, with the cabling of cells within the microconduit. The acceleration of neurite extension was found to be independent of conduit diameter within the range of 200 to 635 microm. Finally, our in vitro model enabled quantification of the effect of combining spatial control and localized nerve growth factor delivery.
Richard G Pearson; Yves Molino; Philip M Williams; Saul J B Tendler; Martyn C Davies; Clive J Roberts; Kevin M Shakesheff
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tissue engineering     Volume:  9     ISSN:  1076-3279     ISO Abbreviation:  Tissue Eng.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-05-12     Completed Date:  2003-07-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9505538     Medline TA:  Tissue Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  201-8     Citation Subset:  IM    
Drug Delivery and Tissue Engineering, School of Pharmaceutical Sciences, University of Nottingham, University Park, United Kingdom.
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MeSH Terms
Collagen Type I
Ganglia, Spinal / cytology*
Image Processing, Computer-Assisted
Implants, Experimental*
Nerve Growth Factor / administration & dosage,  pharmacology
Nerve Regeneration* / drug effects
Neurites / drug effects,  physiology*
Neurons, Afferent / physiology*,  ultrastructure
Rats, Wistar
Time Factors
Tissue Engineering / instrumentation,  methods*
Reg. No./Substance:
0/Collagen Type I; 25852-47-5/Hydrogel; 9061-61-4/Nerve Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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