| Spatial Organization and Stoichiometry of N-Terminal Domain-Mediated Glycosyltransferase Complexes in Golgi Membranes Determined by Fret Microscopy. | |
| | |
MedLine Citation:
|
PMID: 22388569 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The functional link between glycolipid glycosyltransferases (GT) relies on the ability of these proteins to form organized molecular complexes. The organization, stoichiometry and composition of these complexes may impact their sorting properties, sub-Golgi localization, and may determine relative efficiency of GT in different glycolipid biosynthetic pathways. In this work, by using Förster resonance energy transfer microscopy in live CHO-K1 cells, we investigated homo- and hetero-complex formation by different GT as well as their spatial organization and molecular stoichiometry on Golgi membranes. We find that GalNAcT and GalT2 Ntd are able to form hetero-complexes in a 1:2 molar ratio at the trans-Golgi network and that GalT2 but not GalNAcT forms homo-complexes. Also, GalNAcT/GalT2 complexes exhibit a stable behavior reflected by its clustered lateral organization. These results reveals that particular topological organization of GTs may have functional implications in determining the composition of glycolipids in cellular membranes. |
| | |
Authors:
|
Mariana L Ferrari; Guillermo A Gomez; Hugo J F Maccioni |
Related Documents
:
|
20354309 - 2-amino-8-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)imidazo[1,2-a][1,3,5]triazin-4(8h)-... 15534389 - 2-[(e)-(4-chlorophenyl)methyleneamino]-n-(x-methylphenyl)-4,5,6,7-tetrahydro-1-benzothi... 19966439 - 4,6-diamino-2-morpholino-5-nitrosopyrimidine: a polarized molecular structure within hy... 15263129 - 3-acetyl-4-benzoyl-2-methyl-5-phenyl-3,3a-dihydropyrazolo[2,3-c]pyrimidine-7(6h)-thione. 17339729 - 1,17-diphenyl-2,4,6,8,10,12,14,16-octaoxaheptadecane. 20124689 - Clindamycin hydrochloride monohydrate and its ethanol solvate. 11031009 - Ab initio and density functional evaluations of the molecular conformations of beta-car... 22091069 - N,n-bis(diphenyl-phosphan-yl)cyclo-butanamine. 18962269 - Derivatives of 2-thiohydantoin as spectrophotometric analytical reagents. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-3-3 |
Journal Detail:
|
Title: Neurochemical research Volume: - ISSN: 1573-6903 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
|
Created Date: 2012-3-5 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7613461 Medline TA: Neurochem Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Departamento de Química Biológica, Facultad de Ciencias Químicas, Centro de Investigaciones en Química Biológica de Córdoba, CIQUIBIC (UNC-CONICET), Universidad Nacional de Córdoba, Ciudad Universitaria, 5000, Córdoba, Argentina. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Two-photon fluorescent probe for cadmium imaging in cells.
Next Document: Role of nanotechnology in agriculture with special reference to management of insect pests.