| Sparing effect of leptin on liver glycogen stores in rats during the fed-to-fasted transition. | |
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MedLine Citation:
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PMID: 10484368 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of moderate hyperleptinemia ( approximately 20 ng/ml) on liver and skeletal muscle glycogen metabolism was examined in Wistar rats. Animals were studied approximately 90 h after receiving recombinant adenoviruses encoding rat leptin (AdCMV-leptin) or beta-galactosidase (AdCMV-betaGal). Liver and skeletal muscle glycogen levels in the fed and fasted (18 h) states were similar in AdCMV-leptin- and AdCMV-betaGal-treated rats. However, after delivery of a glucose bolus, liver glycogen levels were significantly greater in AdCMV-leptin compared with AdCMV-betaGal rats (P < 0.05). To investigate the mechanism(s) of these differences, glycogen levels were measured immediately after the cessation of a 3- or 6-h glucose infusion or 3, 6, and 9 h after the cessation of a 6-h glucose infusion. Similar increases in liver and skeletal muscle glycogen occurred in hyperleptinemic and control rats in response to glucose infusions. However, 3 and 6 h after the cessation of a glucose infusion, liver glycogen levels were approximately twofold greater (P < 0.05) in AdCMV-leptin-treated compared with AdCMV-betaGal-treated animals. Skeletal muscle glycogen levels were similar in AdCMV-leptin-treated and AdCMV-betaGal-treated animals at the same time points. Glycogen phosphorylase, phosphodiesterase 3B, and glycogen synthase activities were unaltered by hyperleptinemia. We conclude that moderate increases in plasma leptin levels decrease liver glycogen degradation during the fed-to-fasted transition. |
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Authors:
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R M O'Doherty; P R Anderson; A Z Zhao; K E Bornfeldt; C B Newgard |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 277 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1999 Sep |
Date Detail:
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Created Date: 1999-10-28 Completed Date: 1999-10-28 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: E544-50 Citation Subset: IM |
Affiliation:
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Gifford Laboratories for Diabetes Research and Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75235, USA. Odohertyr@msx.dept-med.Pitt.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3',5'-Cyclic-AMP Phosphodiesterases
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metabolism Adipose Tissue / anatomy & histology Animals Body Weight Cyclic Nucleotide Phosphodiesterases, Type 3 Eating / physiology* Epididymis Fasting / physiology* Glycogen / metabolism* Glycogen Synthase / metabolism Leptin / pharmacology* Liver / metabolism* Male Muscle, Skeletal / drug effects, metabolism Organ Size Phosphorylases / metabolism Rats Rats, Wistar beta-Galactosidase / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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P30-DK-17047/DK/NIDDK NIH HHS; P50-H2598801//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Leptin; 9005-79-2/Glycogen; EC 2.4.1.-/Phosphorylases; EC 2.4.1.11/Glycogen Synthase; EC 3.1.4.17/3',5'-Cyclic-AMP Phosphodiesterases; EC 3.1.4.17/Cyclic Nucleotide Phosphodiesterases, Type 3; EC 3.1.4.17/Pde3b protein, rat; EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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