| Spaceflight regulates ryanodine receptor subtype 1 in portal vein myocytes in the opposite way of hypertension. | |
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MedLine Citation:
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PMID: 22096120 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Gravity has a structural role for living systems. Tissue development, architecture and organization are modified when the gravity vector is changed. In particular, microgravity induces a redistribution of blood volume and thus pressure in the astronaut body, abolishing an upright blood pressure gradient inducing orthostatic hypotension. The present study was designed to investigate whether isolated vascular smooth muscle cells are directly sensitive to altered gravitational forces and, secondly, whether sustained blood pressure changes act on the same molecular target. Exposure to microgravity during 8 days in International Space Station induced the decrease of ryanodine receptor subtype 1 expression in primary cultured myocytes from rat hepatic portal vein. Identical results were found in portal vein from mice exposed to microgravity during an 8 days Shuttle spaceflight. To evaluate the functional consequences of this physiological adaptation, we have compared evoked calcium signals obtained in myocytes from hindlimb unloaded rats in which the shift of blood pressure mimics the one produced by the microgravity to those obtained in myocytes from rats injected with antisense oligonucleotide directed against ryanodine receptor subtype 1. In both conditions, calcium signals implicating calcium induced calcium release were significantly decreased. In contrary, in spontaneous hypertensive rat, an increase of ryanodine receptor subtype 1 expression was observed as well as the calcium induced calcium release mechanism. Taken together, our results shown that myocytes were directly sensitive to gravity level and they adapt their calcium signaling pathways to pressure by the regulation of the ryanodine receptor subtype 1 expression. |
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Authors:
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Fabrice Dabertrand; Yves Porte; Nathalie Macrez; Jean-Luc Morel |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-17 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: - ISSN: 1522-1601 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1University of Vermont. |
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