Document Detail

Space flight affects motility and cytoskeletal structures in human monocyte cell line J-111.
MedLine Citation:
PMID:  21246756     Owner:  NLM     Status:  MEDLINE    
Certain functions of immune cells in returning astronauts are known to be altered. A dramatic depression of the mitogenic in vitro activation of human lymphocytes was observed in low gravity. T-cell activation requires the interaction of different type of immune cells as T-lymphocytes and monocytes. Cell motility based on a continuous rearrangement of the cytoskeletal network within the cell is essential for cell-cell contacts. In this investigation on the International Space Station we studied the influence of low gravity on different cytoskeletal structures in adherent monocytes and their ability to migrate. J-111 monocytes were incubated on a colloid gold substrate attached to a cover slide. Migrating cells removed the colloid gold, leaving a track recording cell motility. A severe reduction of the motility of J-111 cells was found in low gravity compared to 1g in-flight and ground controls. Cell shape appeared more contracted, whereas the control cells showed the typical morphology of migrating monocytes, i.e., elongated and with pseudopodia. A qualitative and quantitative analysis of the structures of F-actin, β-tubulin and vinculin revealed that exposure of J-111 cells to low gravity affected the distribution of the different filaments and significantly reduced the fluorescence intensity of F-actin fibers. Cell motility relies on an intact structure of different cytoskeletal elements. The highly reduced motility of monocytes in low gravity must be attributed to the observed severe disruption of the cytoskeletal structures and may be one of the reasons for the dramatic depression of the in vitro activation of human lymphocytes.
Maria Antonia Meloni; Grazia Galleri; Giuseppe Pani; Angela Saba; Proto Pippia; Marianne Cogoli-Greuter
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytoskeleton (Hoboken, N.J.)     Volume:  68     ISSN:  1949-3592     ISO Abbreviation:  Cytoskeleton (Hoboken)     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-19     Completed Date:  2011-04-25     Revised Date:  2014-07-24    
Medline Journal Info:
Nlm Unique ID:  101523844     Medline TA:  Cytoskeleton (Hoboken)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  125-37     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
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MeSH Terms
Actins / metabolism*
Cell Line
Cell Movement*
Cytoskeleton / metabolism*
Monocytes / cytology,  immunology,  metabolism*
Space Flight*
Weightlessness / adverse effects*
Reg. No./Substance:

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