Document Detail

SpMyb functions as an intramodular repressor to regulate spatial expression of CyIIIa in sea urchin embryos.
MedLine Citation:
PMID:  9428408     Owner:  NLM     Status:  MEDLINE    
The CyIIIa actin gene of Strongylocentrotus purpuratus is transcribed exclusively in the embryonic aboral ectoderm, under the control of 2.3 kb cis-regulatory domain that contains a proximal module that controls expression in early embryogenesis, and a middle module that controls expression in later embryogenesis. Previous studies demonstrated that the SpRunt-1 target site within the middle module is required for the sharp increase in CyIIIa transcription which accompanies differentiation of the aboral ectoderm, and that a negative regulatory region near the SpRunt-1 target site is required to prevent ectopic transcription in the oral ectoderm and skeletogenic mesenchyme. This negative regulatory region contains a consensus binding site for the myb family of transcription factors. In vitro DNA-binding experiments reveal that a protein in blastula-stage nuclei interacts specifically with the myb target site. Gene transfer experiments utilizing CyIIIa reporter constructs containing oligonucleotide substitutions indicate that this site is both necessary and sufficient to prevent ectopic expression of CyIIIa. Synthetic oligonucleotides containing the myb target site were used to purify a protein from sea urchin embryo nuclear extracts by affinity chromatography. This protein is immunoprecipitated by antibodies specific to the evolutionarily conserved myb domain, and amino acid sequences obtained from the purified protein were found to be identical to sequences within the myb domain. Sequence information was used to obtain cDNA clones of SpMyb, the S. purpuratus member of the myb family of transcription factors. Through interactions within the middle module, SpMyb functions to repress activation of CyIIIa in the oral ectoderm and skeletogenic mesenchyme.
J A Coffman; C V Kirchhamer; M G Harrington; E H Davidson
Related Documents :
18086838 - Increases in slt2 expression and chitin content are associated with incomplete killing ...
23372488 - Renewing the assault on mrna.
2678098 - Differential binding of nuclear factors to the intron 1 sequences containing the transc...
23408108 - Regulation of aromatase expression in breast cancer treated with anastrozole neoadjuvan...
20126398 - Differential expression of herg1 channel isoforms reproduces properties of native i(kr)...
15115918 - A 6 bp polymorphism in the thymidylate synthase gene causes message instability and is ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  124     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-02-02     Completed Date:  1998-02-02     Revised Date:  2009-07-17    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  4717-27     Citation Subset:  IM    
Stowers Institute for Medical Research, California Institute of Technology, Pasadena 91125, USA.
Data Bank Information
Bank Name/Acc. No.:
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Actins / genetics*,  metabolism
Amino Acid Sequence
Base Sequence
Binding Sites
Chromatography, Affinity
DNA-Binding Proteins / genetics,  metabolism
Embryo, Nonmammalian
Gene Dosage
Gene Expression Regulation, Developmental
Molecular Sequence Data
Oligonucleotides / chemical synthesis
Repressor Proteins / genetics*,  isolation & purification,  metabolism*
Sea Urchins / embryology*,  genetics*
Sequence Homology, Amino Acid
Tissue Distribution
Transcription Factors / genetics*,  isolation & purification,  metabolism*
Grant Support
Reg. No./Substance:
0/Actins; 0/DNA-Binding Proteins; 0/Oligonucleotides; 0/Repressor Proteins; 0/SpRunt-1 protein, Strongylocentrotus purpuratus; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Bicalutamide (Casodex) in the treatment of prostate cancer: history of clinical development.
Next Document:  MyoD and Myf-5 differentially regulate the development of limb versus trunk skeletal muscle.