Document Detail

Soybean lipoxygenase catalysed oxygenation of unsaturated fatty acid encapsulated in cyclodextrin.
MedLine Citation:
PMID:  1903659     Owner:  NLM     Status:  MEDLINE    
The linoleic or arachidonic acid entrapped in cyclodextrin (alpha, beta or gamma) serves as an excellent substrate for soybean lipoxygenase-1 catalysis. At pH 9.0 the Km values for the beta-cyclodextrin encapsulated arachidonic acid, referred herein as encapsulated substrate, and the Tween-20 dispersed substrate were 7.7 microM and 7.5 microM, respectively. However, the Vmax values for alpha- and beta-cyclodextrin solubilized substrates were lower in comparison with the Tween-20 dispersed substrate. Interestingly, the pH-activity profile for the enzyme towards cyclodextrin encapsulated arachidonic acid showed optimum around 7.5, while that towards Tween-20 dispersion showed the expected broad optimum in the alkaline range (8.5-10.0). The activity with encapsulated substrate at pH 7.5 was at least 5-fold higher than that obtained with Tween-20 dispersed substrate at the corresponding pH. Similar results were obtained using linoleic acid. The second order rate constant, Kcat/Km, for the encapsulated substrate was an order of magnitude higher when compared to the Tween-20 dispersed substrate. The plot of v obtained at pH 9.0, against S gave hyperbolic curves for both the encapsulated as well as the Tween-20 dispersed substrates, whereas at pH 7.5, the curve for cyclodextrin encapsulated arachidonic acid appeared initially concave and then at higher concentrations of the substrate sigmoidal. The positional specificity of soybean lipoxygenase remained unaltered, however.
N Jyothirmayi; C S Ramadoss
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1083     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1991 May 
Date Detail:
Created Date:  1991-07-02     Completed Date:  1991-07-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  193-200     Citation Subset:  IM    
Food Chemistry Department, Central Food Technological Research Institute, Mysore, India.
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MeSH Terms
Arachidonic Acid
Arachidonic Acids / metabolism
Chromatography, High Pressure Liquid
Fatty Acids, Unsaturated / metabolism*
Hydrogen-Ion Concentration
Linoleic Acid
Linoleic Acids / metabolism
Lipoxygenase / metabolism*
Solanum tuberosum / enzymology
Soybeans / enzymology*
Reg. No./Substance:
0/Arachidonic Acids; 0/Capsules; 0/Cyclodextrins; 0/Fatty Acids, Unsaturated; 0/Linoleic Acids; 0/Polysorbates; 2197-37-7/Linoleic Acid; 506-32-1/Arachidonic Acid; EC

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