Document Detail


Soyasaponins inhibit the proliferation of Hela cells by inducing apoptosis.
MedLine Citation:
PMID:  17582751     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This research aimed at investigating the effect of soyasaponins on the proliferation of Hela cells. SS-II, the second fraction of soyasaponins, was separated by column chromatographic method with D101A macroporous resin from soybeans. SS-II could inhibit the proliferation of Hela cells by changing cell cycle distribution and inducing apoptosis. Furthermore, the loss of mitochondrial transmembrane potential was observed by flow cytometry and the increase of intracellular Ca(2+) concentration was detected by confocal laser scanning microscope in apoptotic cells. At the same time, the nitric oxide content, nitric oxide synthase (NOS) and inducible NOS activities were also increased. The above results suggested that the inhibitory effect of SS-II on Hela cell proliferation was caused by inducing apoptosis through the mitochondrial pathway.
Authors:
Jun-Xia Xiao; Guo-Qing Huang; Sheng-Hua Zhang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-20
Journal Detail:
Title:  Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie     Volume:  59     ISSN:  0940-2993     ISO Abbreviation:  Exp. Toxicol. Pathol.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-16     Completed Date:  2007-10-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208920     Medline TA:  Exp Toxicol Pathol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  35-42     Citation Subset:  IM    
Affiliation:
School of Food Science and Engineering, Laiyang Agricultural University, Qingdao, China.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents, Phytogenic / pharmacology*
Apoptosis / drug effects*
Calcium / metabolism
Cell Cycle / drug effects
Cell Proliferation / drug effects*
Drug Screening Assays, Antitumor
Female
Hela Cells
Humans
Membrane Potential, Mitochondrial / drug effects
Microscopy, Confocal
Nitric Oxide / metabolism
Nitric Oxide Synthase Type II / metabolism
Oleanolic Acid / analogs & derivatives*,  pharmacology
Saponins / pharmacology*
Soybeans / chemistry*
Uterine Cervical Neoplasms / drug therapy*,  metabolism,  pathology
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Saponins; 10102-43-9/Nitric Oxide; 508-02-1/Oleanolic Acid; 55319-36-3/soyasaponin II; 7440-70-2/Calcium; EC 1.14.13.39/Nitric Oxide Synthase Type II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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