Document Detail

Soy isoflavonoids exhibit in vitro biological activities of loop diuretics.
MedLine Citation:
PMID:  9848498     Owner:  NLM     Status:  MEDLINE    
Intake of soyfoods containing isoflavonoids is related to positive effects on heart and kidney diseases. Urinary equol, a potent inhibitor of Na+-K+-2Cl- cotransport, originates from the metabolism of daidzein by intestinal bacteria. Loop diuretics (eg, furosemide), acting through inhibition of Na+-K+-2Cl- cotransport, are used to maintain adequate blood volume. In the present work, we compare isoflavonoids' inhibition of cotransport and effects on the function and hemodynamics of isolated perfused rat kidneys with those of furosemide. Equol [IC50 (half-maximal inhibitory concentration): 23.6 +/- 3.6 micromol/L], genistein (IC50: 34.8 +/- 2.6 micromol/L), and daidzein (IC50: 140.0 +/- 24 micromol/L) inhibited bumetanide-sensitive rubidium uptake in LLC-PK1 cells. The IC50 of equol and genistein was close to that of furosemide (IC50: 10.3 +/- 2.7 micromol/L). Furosemide, equol, and genistein stimulated water, sodium, and potassium excretion by isolated rat kidneys in the same temporal pattern. None of the isoflavonoids significantly increased the glomerular filtration rate, but genistein induced significant vasorelaxation. We conclude that isoflavonoids exhibit biological activities of furosemide in vitro, at concentrations similar to those reported for other in vitro effects. More research is needed to evaluate the participation of cotransport inhibition by isoflavonoids in the healthful effects claimed for soy intake.
R M Martínez; I Giménez; J M Lou; J A Mayoral; J O Alda
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of clinical nutrition     Volume:  68     ISSN:  0002-9165     ISO Abbreviation:  Am. J. Clin. Nutr.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-01-06     Completed Date:  1999-01-06     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0376027     Medline TA:  Am J Clin Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1354S-1357S     Citation Subset:  AIM; IM    
Department of Pharmacology and Physiology, Faculty of Medicine, University of Zaragoza, Spain.
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MeSH Terms
Chromans / pharmacology
Diuresis / drug effects
Diuretics / pharmacology*
Enzyme Inhibitors / pharmacology
Furosemide / pharmacology*
Genistein / pharmacology
Glomerular Filtration Rate / drug effects
Hemodynamics / drug effects
Ion Transport / drug effects*
Isoflavones / pharmacology*
Kidney / drug effects*
Monoamine Oxidase Inhibitors / pharmacology
Natriuresis / drug effects
Soybean Proteins / pharmacology*
Reg. No./Substance:
0/Chromans; 0/Diuretics; 0/Enzyme Inhibitors; 0/Isoflavones; 0/Monoamine Oxidase Inhibitors; 0/Soybean Proteins; 446-72-0/Genistein; 531-95-3/equol; 54-31-9/Furosemide

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