| Soy isoflavone and its effect to regulate hypothalamus and peripheral orexigenic gene expression in ovariectomized rats fed on a high-fat diet. | |
| | |
MedLine Citation:
|
PMID: 20486439 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: To explore the effect of soy isoflavone on obesity in the light of hypothalamus and peripheral orexigenic gene regulation. METHODS: Fifty-four female rats were randomly assigned to 6 groups: one sham-operated group (SHAM), one ovariectomized (OVX) control group, three OVX groups fed with 400 ppm (L-SI), 1200 ppm (M-SI) and 3600 ppm (H-SI) isoflavone respectively, and one OVX group receiving 0.45 ppm diethylstilbestrol (EC). All rats were allowed to take high-fat diet for 4 weeks. Some neuropeptides were measured by RT-PCR. These neuropeptides included NPY, pro-opiomelanocortin (POMC), cocaine and amphetamine regulated transcript (CART), orexin, melanin-concentrating hormone (MCH), melanin-concentrating hormone precursor (P-MCH), ghrelin, and leptin. RESULTS: Compared with the OVX control group, the body weight and food intake in the H-SI group were reduced significantly and there was a significant dose-dependent manner in the 3 isoflavone groups. The results of RT-PCR showed that the NPY level in the 3 isoflavone groups was significantly increased and the POMC/CART gene expression decreased significantly in rats' hypothalamus compared with that in the OVX control group. However, the expression of orexin, MCH and P-MCH had no change. The peripheral grelin mRNA expression was higher in the 3 isoflavone groups, while leptin gene expression in the fat was not consistent. CONCLUSIONS: This research showed that isoflavone could prevent obesity induced by high-fat diet and ovariectomy through regulating hypothalamus and peripheral orexigenic gene expressions associated with food intake. |
| | |
Authors:
|
Yun-Bo Zhang; Yang Zhang; Li-Na Li; Xin-Yu Zhao; Xiao-Lin Na |
Related Documents
:
|
9252929 - Estrogen-androgen for hormone replacement. a review. 11999549 - Beneficial effects of soybean isoflavone supplementation on bone metabolism and serum l... 12747219 - Potential risks and benefits of phytoestrogen-rich diets. 12081819 - A comparison of the effects of 2 doses of soy protein or casein on serum lipids, serum ... 14642739 - Effects of dietary soy and estrous cycle on adrenal cytochrome p450 1b1 expression and ... 17069349 - Soy protein containing isoflavones favorably influences macrophage lipoprotein metaboli... 18679589 - Trace metal concentrations in the hair of zalophus californianus pups and their relatio... 18176229 - Some anatomical observations on midface aging and long-term results of surgical treatment. 7825519 - Copper deficiency: interaction with high-fructose and high-fat diets in rats. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biomedical and environmental sciences : BES Volume: 23 ISSN: 0895-3988 ISO Abbreviation: Biomed. Environ. Sci. Publication Date: 2010 Feb |
Date Detail:
|
Created Date: 2010-05-21 Completed Date: 2010-06-15 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8909524 Medline TA: Biomed Environ Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 68-75 Citation Subset: IM |
Affiliation:
|
Department of Environmental Hygiene, College of Public Health, Harbin Medical University, Harbin 150086, Heilongjiang, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Dietary Fats / pharmacology* Feeding Behavior / drug effects, physiology Female Gene Expression Regulation / drug effects* Hypothalamus Isoflavones / chemistry*, pharmacology* Neuropeptides / genetics, metabolism* Obesity Ovariectomy RNA, Messenger / genetics, metabolism Rats Soybeans / chemistry* |
| Chemical | |
Reg. No./Substance:
|
0/Dietary Fats; 0/Isoflavones; 0/Neuropeptides; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Combined transplantation of neural stem cells and olfactory ensheathiny cells improves the motor fun...
Next Document: Preparation of polyclonal antibody against human MxA protein and its specificity to diversified myxo...