Document Detail

Soy isoflavone and its effect to regulate hypothalamus and peripheral orexigenic gene expression in ovariectomized rats fed on a high-fat diet.
MedLine Citation:
PMID:  20486439     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To explore the effect of soy isoflavone on obesity in the light of hypothalamus and peripheral orexigenic gene regulation. METHODS: Fifty-four female rats were randomly assigned to 6 groups: one sham-operated group (SHAM), one ovariectomized (OVX) control group, three OVX groups fed with 400 ppm (L-SI), 1200 ppm (M-SI) and 3600 ppm (H-SI) isoflavone respectively, and one OVX group receiving 0.45 ppm diethylstilbestrol (EC). All rats were allowed to take high-fat diet for 4 weeks. Some neuropeptides were measured by RT-PCR. These neuropeptides included NPY, pro-opiomelanocortin (POMC), cocaine and amphetamine regulated transcript (CART), orexin, melanin-concentrating hormone (MCH), melanin-concentrating hormone precursor (P-MCH), ghrelin, and leptin. RESULTS: Compared with the OVX control group, the body weight and food intake in the H-SI group were reduced significantly and there was a significant dose-dependent manner in the 3 isoflavone groups. The results of RT-PCR showed that the NPY level in the 3 isoflavone groups was significantly increased and the POMC/CART gene expression decreased significantly in rats' hypothalamus compared with that in the OVX control group. However, the expression of orexin, MCH and P-MCH had no change. The peripheral grelin mRNA expression was higher in the 3 isoflavone groups, while leptin gene expression in the fat was not consistent. CONCLUSIONS: This research showed that isoflavone could prevent obesity induced by high-fat diet and ovariectomy through regulating hypothalamus and peripheral orexigenic gene expressions associated with food intake.
Yun-Bo Zhang; Yang Zhang; Li-Na Li; Xin-Yu Zhao; Xiao-Lin Na
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomedical and environmental sciences : BES     Volume:  23     ISSN:  0895-3988     ISO Abbreviation:  Biomed. Environ. Sci.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-05-21     Completed Date:  2010-06-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8909524     Medline TA:  Biomed Environ Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  68-75     Citation Subset:  IM    
Department of Environmental Hygiene, College of Public Health, Harbin Medical University, Harbin 150086, Heilongjiang, China.
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MeSH Terms
Dietary Fats / pharmacology*
Feeding Behavior / drug effects,  physiology
Gene Expression Regulation / drug effects*
Isoflavones / chemistry*,  pharmacology*
Neuropeptides / genetics,  metabolism*
RNA, Messenger / genetics,  metabolism
Soybeans / chemistry*
Reg. No./Substance:
0/Dietary Fats; 0/Isoflavones; 0/Neuropeptides; 0/RNA, Messenger

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