Document Detail

Soy Lecithin Interferes with Mitochondrial Function in Frozen-Thawed Ram Spermatozoa.
MedLine Citation:
PMID:  22134371     Owner:  NLM     Status:  Publisher    
Egg yolk and milk are the two major membrane cryoprotectants commonly used in freezing media for the long-term preservation of semen (alone or in combination with others). However, in recent years there have been increasing arguments against the presence of egg yolk or milk, due to the risk of introducing diseases through the use of cryopreserved semen. In this study, we have analysed the protective effect of lecithin as an alternative to egg-yolk for the cryopreservation of ram semen, using a range of functional markers for sperm viability, motility, apoptosis, mitochondrial functionality analyses (mitochondrial inner membrane surface, MIMS; mitochondrial inner membrane potential, MIMP; and cell membrane potential, CMP) as methods of assessment in samples diluted in 3 different media: Tris-citrate-glucose as control, and two media supplemented with soy lecithin or egg yolk. The results showed that lecithin is able to effectively protect certain sperm quality characteristics against freezing-induced damage. However, lecithin induced loss of mitochondrial membrane potential or mitochondrial loss that, in fresh semen, was not reflected by modifications in sperm motility. MIMS and MIMP values decreased in thawed lecithin-treated samples, concomitant with a lower (p<0.05) percentage of total and progressively motile cells than those in egg yolk-containing samples. Further incubation of thawed samples revealed changes in motility and mitochondrial functionality that, otherwise, would not have been detected. These results indicate that lecithin may have affected the inner mitochondrial membrane in frozen-thawed spermatozoa, and confirm that sublethal damages that seriously affect sperm functionality, not detected by classical sperm quality analyses, can be evidenced by changes in the inner mitochondrial membrane surface. These findings strengthen the relationship between mitochondrial membrane potential and motility, and show that the mitochondrial alterations induced by the cryopreservation process could be specific targets for the improvement of semen cryopreservation protocols.
I Del Valle; A Gómez-Durán; W V Holt; T Muiño-Blanco; J A Cebrián-Pérez
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-1
Journal Detail:
Title:  Journal of andrology     Volume:  -     ISSN:  1939-4640     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8106453     Medline TA:  J Androl     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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