Document Detail


Soy-based diet exacerbates seizures in mouse models of neurological disease.
MedLine Citation:
PMID:  23034522     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Seizures are a common phenotype in many neurological disorders including Alzheimer's disease, Down syndrome, and fragile X syndrome. Mouse models of these disorders overexpress amyloid-β protein precursor (AβPP) and amyloid-β (Aβ) and are highly susceptible to audiogenic-induced seizures (AGS). We observed decreased AGS in these mice fed a casein-based, purified diet (D07030301) as opposed to a standard soy protein-containing, non-purified diet (Purina 5015). Our objective in this manuscript was to determine if soy protein, and in particular soy isoflavones, in the Purina 5015 were contributing to the seizure phenotype. Wild running, AGS, and death rates were assessed in juvenile mice fed Purina 5015, D07030301, D07030301 containing soy protein, or D07030301 supplemented with individual isoflavones (750 mg/kg daidzein or genistein). A short treatment (3 days) with Purina 5015 induced wild running and AGS in Alzheimer's disease mice. A 3-day treatment with daidzein-supplemented diet, but not genistein, induced wild running in wild type mice. To understand the mechanism underlying daidzein activity, we assessed dendritic AβPP expression in primary, cultured, wild type neurons treated with daidzein or genistein. In vitro, daidzein significantly increased dendritic AβPP. Thus, the soy isoflavone daidzein recapitulated seizure induction in vivo and altered AβPP expression in vitro. These results have important implications for individuals on soy-based diets as well as for rodent model research.
Authors:
Cara J Westmark; Pamela R Westmark; James S Malter
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of Alzheimer's disease : JAD     Volume:  33     ISSN:  1875-8908     ISO Abbreviation:  J. Alzheimers Dis.     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-14     Completed Date:  2013-06-25     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  9814863     Medline TA:  J Alzheimers Dis     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  797-805     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acoustic Stimulation / adverse effects
Amyloid beta-Protein Precursor / genetics,  metabolism
Animals
Cells, Cultured
Cerebral Cortex / cytology
Diet*
Disease Models, Animal
Embryo, Mammalian
Female
Fragile X Mental Retardation Protein / genetics
Humans
Locomotion / genetics
Male
Mice
Mice, Transgenic
Nervous System Diseases / complications*,  genetics,  mortality
Pregnancy
Presenilin-1 / genetics
Seizures / diet therapy,  etiology*
Soybean Proteins / adverse effects*,  chemistry
Grant Support
ID/Acronym/Agency:
1UL1RR025011/RR/NCRR NIH HHS; 9U54TR000021/TR/NCATS NIH HHS; P30 HD003352/HD/NICHD NIH HHS; P30 HD03352/HD/NICHD NIH HHS; UL1 RR025011/RR/NCRR NIH HHS; UL1 TR000427/TR/NCATS NIH HHS
Chemical
Reg. No./Substance:
0/Amyloid beta-Protein Precursor; 0/Fmr1 protein, mouse; 0/PSEN1 protein, human; 0/Presenilin-1; 0/Soybean Proteins; 139135-51-6/Fragile X Mental Retardation Protein
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Longitudinal Stability of Cerebrospinal Fluid Biomarker Levels: Fulfilled Requirement for Pharmacody...
Next Document:  Clinically asymptomatic vascular brain injury: a potent cause of cognitive impairment among older in...