Document Detail

Sox2 up-regulation and glial cell proliferation following degeneration of spiral ganglion neurons in the adult mouse inner ear.
MedLine Citation:
PMID:  21061038     Owner:  NLM     Status:  MEDLINE    
In the present study, glial cell responses to spiral ganglion neuron (SGN) degeneration were evaluated using a murine model of auditory neuropathy. Ouabain, a well-known Na,K-ATPase inhibitor, has been shown to induce SGN degeneration while sparing hair cell function. In addition to selectively removing type I SGNs, ouabain leads to hyperplasia and hypertrophy of glia-like cells in the injured auditory nerves. As the transcription factor Sox2 is predominantly expressed in proliferating and undifferentiated neural precursors during neurogenesis,we sought to examine Sox2 expression patterns following SGN injury by ouabain. Real-time RT-PCR and Western blot analyses of cochlea indicated a significant increase in Sox2 expression by 3 days posttreatment with ouabain. Cells incorporating bromodeoxyuridine(BrdU) and expressing Sox2 were counted in the auditory nerves of control and ouabain-treated ears. The glial phenotype of Sox2+cells was identified by two neural glial markers: S100 and Sox10. The number of Sox2+ glial cells significantly increased at 3 days post-treatment and reached its maximum level at 7 days post-treatment. Similarly,the number of BrdU+ cells increased at 3 and 7 days post-treatment in the injured nerves. Quantitative analysis with dual-immunostaining procedures indicated that about 70% of BrdU+ cells in the injured nerves were Sox2+ glial cells. These results demonstrate that up-regulation of Sox2 expression is associated with increased cell proliferation in the auditory nerve after injury.
Hainan Lang; Manna Li; Lauren A Kilpatrick; Juhong Zhu; Devadoss J Samuvel; Edward L Krug; John C Goddard
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of the Association for Research in Otolaryngology : JARO     Volume:  12     ISSN:  1438-7573     ISO Abbreviation:  J. Assoc. Res. Otolaryngol.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-06     Completed Date:  2011-06-16     Revised Date:  2014-05-05    
Medline Journal Info:
Nlm Unique ID:  100892857     Medline TA:  J Assoc Res Otolaryngol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  151-71     Citation Subset:  IM    
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MeSH Terms
Cell Proliferation*
Cochlear Nerve / drug effects,  metabolism,  pathology
Disease Models, Animal
Ear, Inner / innervation*
Enzyme Inhibitors / adverse effects,  pharmacology
Hearing Loss, Central / metabolism,  pathology
Mice, Inbred CBA
Nerve Degeneration / chemically induced,  metabolism*,  pathology*
Neuroglia / metabolism*,  pathology*
Ouabain / adverse effects,  pharmacology
SOXB1 Transcription Factors / metabolism*
Spiral Ganglion / drug effects,  metabolism,  pathology
Up-Regulation / physiology
Grant Support
Reg. No./Substance:
0/Enzyme Inhibitors; 0/SOXB1 Transcription Factors; 0/Sox2 protein, mouse; 5ACL011P69/Ouabain

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