Document Detail

Sox14 is required for transcriptional and developmental responses to 20-hydroxyecdysone at the onset of drosophila metamorphosis.
MedLine Citation:
PMID:  20803583     Owner:  NLM     Status:  MEDLINE    
The steroid hormone 20-hydroxyecdysone (20E), by means of a heterodimer consisting of two nuclear receptors, the Ecdysone receptor (EcR) and Ultraspiracle (Usp), triggers the major developmental transitions in the Drosophila life cycle through the regulation of genetic hierarchies. We have previously demonstrated that the Sox14 transcription factor is a primary response gene to 20E/EcR/Usp complex. In this study, we show that mutations in sox14 result in prepupal and pupal lethality with animals displaying a multitude of defects in 20E developmentally regulated pathways. In addition, through Northern blot and microarray analyses of sox14 mutant animals, we demonstrate that Sox14 is required for the proper expression of 20E- and non-20E-regulated genes at the onset of metamorphosis. We also show that the Sox14-regulated gene set correlates well with Sox14 expression in a variety of larval and adult tissues. Thus, Sox14 is a critical transcription factor required for 20E signaling at the onset of metamorphosis.
Amanda R Ritter; Robert B Beckstead
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  239     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-11-01     Completed Date:  2011-03-15     Revised Date:  2011-11-10    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2685-94     Citation Subset:  IM    
Department of Poultry Science, The University of Georgia, Athens, Georgia 30602, USA.
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MeSH Terms
Blotting, Northern
DNA-Binding Proteins / genetics,  metabolism
Drosophila Proteins / genetics,  metabolism*
Ecdysterone / metabolism*
Gene Expression Regulation, Developmental / genetics,  physiology*
Metamorphosis, Biological / genetics*
Oligonucleotide Array Sequence Analysis
Receptors, Steroid / genetics,  metabolism
SOXB2 Transcription Factors / genetics,  metabolism*
Transcription Factors / genetics,  metabolism
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Drosophila Proteins; 0/Receptors, Steroid; 0/SOXB2 Transcription Factors; 0/Sox14 protein, Drosophila; 0/Transcription Factors; 0/ecdysone receptor; 0/ultraspiracle protein, Drosophila; 5289-74-7/Ecdysterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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