Document Detail

Sources of clostridia in raw milk on farms.
MedLine Citation:
PMID:  18757576     Owner:  NLM     Status:  MEDLINE    
A PCR-denaturing gradient gel electrophoresis (DGGE) method was used to examine on-farm sources of Clostridium cluster I strains in four dairy farms over 2 years. Conventional microbiological analysis was used in parallel to monitor size of clostridial populations present in various components of the milk production chain (soil, forage, grass silage, maize silage, dry hay, and raw milk). PCR amplification with Clostridium cluster I-specific 16S rRNA gene primers followed by DGGE separation yielded a total of 47 operational taxonomic units (OTUs), which varied greatly with respect to frequency of occurrence. Some OTUs were found only in forage, and forage profiles differed according to farm location (southern or northern Québec). More clostridial contamination was found in maize silage than in grass silage. Milk represented a potential environment for certain OTUs. No OTU was milk specific, indicating that OTUs originated from other environments. Most (83%) of the OTUs detected in raw milk were also found in grass or maize silage. Milk DGGE profiles differed according to farm and sampling year and fit into two distinct categories. One milk profile category was characterized by the presence of a few dominant OTUs, the presence of which appeared to be more related to farm management than to feed contamination. OTUs were more varied in the second profile category. The identities of certain OTUs frequently found in milk were resolved by cloning and sequencing. Clostridium disporicum was identified as an important member of clostridial populations transmitted to milk. Clostridium tyrobutyricum was consistently found in milk and was widespread in the other farm environments examined.
Marie-Claude Julien; Patrice Dion; Carole Lafrenière; Hani Antoun; Pascal Drouin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-29
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  74     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-14     Completed Date:  2008-11-18     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6348-57     Citation Subset:  IM    
Département de phytologie, Pavillon Charles-Eugène Marchand, Université Laval, Québec, Québec, Canada.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EU478466;  EU478467;  EU478468;  EU478469;  EU478470;  EU478471;  EU478472;  EU478473;  EU478474;  EU478475;  EU478476;  EU478477;  EU478478;  EU478479;  EU478480;  EU478481;  EU478482;  EU478483;  EU478484;  EU478485;  EU478486;  EU478487;  EU478488
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MeSH Terms
Clostridium / classification*,  isolation & purification*
Cluster Analysis
DNA Fingerprinting
DNA, Bacterial / chemistry,  genetics
DNA, Ribosomal / chemistry,  genetics
Electrophoresis, Polyacrylamide Gel / methods
Environmental Microbiology*
Food Contamination*
Genes, rRNA
Milk / microbiology*
Molecular Epidemiology
Molecular Sequence Data
Nucleic Acid Denaturation
Polymerase Chain Reaction
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Bacterial; 0/RNA, Ribosomal, 16S

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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