Document Detail


Sorting of the v-SNARE VAMP7 in Dictyostelium discoideum: a role for more than one Adaptor Protein (AP) complex.
MedLine Citation:
PMID:  18634783     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Soluble N-ethylmaleimide-sensitive-factor Attachment protein Receptors (SNAREs) participate in the specificity of membrane fusions in the cell. The mechanisms of specific SNARE sorting are still however poorly documented. We investigated the possible role of Adaptor Protein (AP) complexes in sorting of the Dictyostelium discoideum v-SNARE VAMP7. In live cells, GFP-VAMP7 is observed in the membrane of endocytic compartments. It is also observed in the plasma membrane of a small proportion of the cells. Mutation of a potential dileucine motif dramatically increases the proportion of cells with GFP-VAMP7 in their plasma membrane, strongly supporting the participation of an AP complex in VAMP7 sorting to the endocytic pathway. A partial increase occurs in knockout cells for the medium subunits of AP-2 and AP-3 complexes, indicating a role for both AP-2 and AP-3. VAMP7, as well as its t-SNAREs partners syntaxin 8 and Vti1, are co-immunoprecipitated with each of the medium subunits of the AP-1, AP-2, AP-3 and AP-4 complexes. This result supports the conclusion that VAMP7 directly interacts with both AP-2 and AP-3. It also raises the hypothesis of an interaction with AP-1 and AP-4. GFP-VAMP7 is retrieved from the endocytic pathway at and/or before the late post-lysosomal stage through an AP-independent mechanism.
Authors:
Nelly Bennett; François Letourneur; Michel Ragno; Mathilde Louwagie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-02
Journal Detail:
Title:  Experimental cell research     Volume:  314     ISSN:  1090-2422     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-01     Completed Date:  2008-10-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2822-33     Citation Subset:  IM    
Affiliation:
Commissariat à l'Energie Atomique, iRTSV, Laboratoire de Biochimie et Biophysique des Systèmes Intégrés, CNRS UMR5092, Université Joseph Fourier, 38054 Grenoble, France. nelly.bennett@cea.fr
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MeSH Terms
Descriptor/Qualifier:
Adaptor Protein Complex 2 / metabolism
Adaptor Proteins, Vesicular Transport / metabolism*
Animals
Cell Membrane / metabolism,  ultrastructure
Dictyostelium / metabolism*
Endocytosis / physiology*
Green Fluorescent Proteins / genetics
Intracellular Membranes / metabolism,  ultrastructure
Macromolecular Substances / metabolism
Protein Transport / physiology
Protozoan Proteins / metabolism
R-SNARE Proteins / metabolism*
Recombinant Fusion Proteins / genetics,  metabolism
SNARE Proteins / metabolism*
Signal Transduction / physiology
Transport Vesicles / metabolism*,  ultrastructure
Chemical
Reg. No./Substance:
0/Adaptor Protein Complex 2; 0/Adaptor Proteins, Vesicular Transport; 0/Macromolecular Substances; 0/Protozoan Proteins; 0/R-SNARE Proteins; 0/Recombinant Fusion Proteins; 0/SNARE Proteins; 147336-22-9/Green Fluorescent Proteins

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