| Sorting of the v-SNARE VAMP7 in Dictyostelium discoideum: a role for more than one Adaptor Protein (AP) complex. | |
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MedLine Citation:
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PMID: 18634783 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Soluble N-ethylmaleimide-sensitive-factor Attachment protein Receptors (SNAREs) participate in the specificity of membrane fusions in the cell. The mechanisms of specific SNARE sorting are still however poorly documented. We investigated the possible role of Adaptor Protein (AP) complexes in sorting of the Dictyostelium discoideum v-SNARE VAMP7. In live cells, GFP-VAMP7 is observed in the membrane of endocytic compartments. It is also observed in the plasma membrane of a small proportion of the cells. Mutation of a potential dileucine motif dramatically increases the proportion of cells with GFP-VAMP7 in their plasma membrane, strongly supporting the participation of an AP complex in VAMP7 sorting to the endocytic pathway. A partial increase occurs in knockout cells for the medium subunits of AP-2 and AP-3 complexes, indicating a role for both AP-2 and AP-3. VAMP7, as well as its t-SNAREs partners syntaxin 8 and Vti1, are co-immunoprecipitated with each of the medium subunits of the AP-1, AP-2, AP-3 and AP-4 complexes. This result supports the conclusion that VAMP7 directly interacts with both AP-2 and AP-3. It also raises the hypothesis of an interaction with AP-1 and AP-4. GFP-VAMP7 is retrieved from the endocytic pathway at and/or before the late post-lysosomal stage through an AP-independent mechanism. |
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Authors:
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Nelly Bennett; François Letourneur; Michel Ragno; Mathilde Louwagie |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-07-02 |
Journal Detail:
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Title: Experimental cell research Volume: 314 ISSN: 1090-2422 ISO Abbreviation: Exp. Cell Res. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-09-01 Completed Date: 2008-10-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: United States |
Other Details:
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Languages: eng Pagination: 2822-33 Citation Subset: IM |
Affiliation:
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Commissariat à l'Energie Atomique, iRTSV, Laboratoire de Biochimie et Biophysique des Systèmes Intégrés, CNRS UMR5092, Université Joseph Fourier, 38054 Grenoble, France. nelly.bennett@cea.fr |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptor Protein Complex 2
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metabolism Adaptor Proteins, Vesicular Transport / metabolism* Animals Cell Membrane / metabolism, ultrastructure Dictyostelium / metabolism* Endocytosis / physiology* Green Fluorescent Proteins / genetics Intracellular Membranes / metabolism, ultrastructure Macromolecular Substances / metabolism Protein Transport / physiology Protozoan Proteins / metabolism R-SNARE Proteins / metabolism* Recombinant Fusion Proteins / genetics, metabolism SNARE Proteins / metabolism* Signal Transduction / physiology Transport Vesicles / metabolism*, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Adaptor Protein Complex 2; 0/Adaptor Proteins, Vesicular Transport; 0/Macromolecular Substances; 0/Protozoan Proteins; 0/R-SNARE Proteins; 0/Recombinant Fusion Proteins; 0/SNARE Proteins; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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