Document Detail


Sorghum, a healthy and gluten-free food for celiac patients as demonstrated by genome, biochemical and immunochemical analyses.
MedLine Citation:
PMID:  23432128     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Wheat (Triticum spp. L.), rye (Secale cereal L.) and barley (Hordeum vulgare L.) seeds contain toxic peptides to celiac patients. Maize (Zea mays L.) and rice (Oryza sativa L.) are distant relatives of wheat as well as Sorghum (Sorghum bicolor (L.) Moench) and are known to be safe for celiacs. Both immunochemical studies and in vitro and in vivo challenge of wheat-free sorghum food products support this conclusion although molecular evidence is missing. The goal of the present study was to provide a biochemical and genetic evidence that sorghum is safe for celiac patients. In silico analysis of the recently published sorghum genome predicts that sorghum does not contain peptides that are toxic for celiac patients. Aqueous/alcohol-soluble prolamins (kafirins) from different sorghum varieties, including pure lines and hybrids, were evaluated by SDS-PAGE and HPLC analyses as well as an established enzyme-linked immunosorbent assay (ELISA) basing on the R5 antibody. These analyses provide molecular evidence for the absence of toxic gliadin-like peptides in sorghum confirming that sorghum can be definitively considered safe for consumption by people with celiac disease.
Authors:
Paola Pontieri; Gianfranco Mamone; Salvatore De Caro; Mitchell R Tuinstra; Earl Roemer; Josephine Okot; Pasquale De Vita; Donatella Bm Ficco; Pietro Alifano; Domenico Pignone; Domenica R Massardo; Luigi Del Giudice
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-22
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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