| Sorbitol transport in rat renal inner medullary interstitial cells. | |
| | |
MedLine Citation:
|
PMID: 11918747 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Sorbitol plays an important role in renal osmoregulation. In the rat renal inner medulla sorbitol synthesis and sorbitol degradation are located in different cell types. Whereas sorbitol synthesis can be detected in the inner medullary collecting duct cells, sorbitol degradation takes place in the interstitial cells. Therefore, one can speculate that the cooperation between epithelial and interstitial cells requires sorbitol transport into interstitial cells. METHODS: Our studies were performed with an interstitial cell line derived from the renal inner medulla of Wistar rats. These cells have typical characteristics of renal fibroblasts. In addition, they possess a high activity of sorbitol dehydrogenase as determined in vivo. Uptake was measured by liquid scintillation counting. For studies on sorbitol metabolism sorbitol concentration was measured photometrically. RESULTS: The results show that sorbitol transport into interstitial cells occurs via a yet to be described transport system. No saturation of sorbitol transport could be found up to an extracellular sorbitol concentration of 80 mmol/L. The transport was neither sodium nor chloride dependent. Trans-stimulation increased the sorbitol uptake. Sorbitol uptake was less inhibited by cytochalasin B than 2-deoxy-D-glucose uptake. The transport showed a high affinity for sorbitol and only little inhibition of sorbitol uptake by substances with a similar structure was observed. CONCLUSIONS: Our results show a new sorbitol transport system in renal inner medullary interstitial cells, which is rather different from the described sorbitol permease in renal epithelial cells and from glucose transporters of the GLUT- and SGLT-family. |
| | |
Authors:
|
Jan B Schüttert; G Martin Fiedler; Clemens Grupp; Sabine Blaschke; R Willi Grunewald |
Related Documents
:
|
20465997 - The effect of folate status on the uptake of physiologically relevant compounds by caco... 9306017 - Choline transport and its osmotic regulation in renal cells derived from the rabbit out... 17937927 - A novel cell penetrating aspartic protease inhibitor blocks processing and presentation... 10874157 - The role of anion exchange in the uptake of pb by human erythrocytes and madin-darby ca... 12374627 - A role for the myoglobin redox cycle in the induction of endothelial cell apoptosis. 6739137 - Eimeria tenella: in vitro development in irradiated bovine kidney cells. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Kidney international Volume: 61 ISSN: 0085-2538 ISO Abbreviation: Kidney Int. Publication Date: 2002 Apr |
Date Detail:
|
Created Date: 2002-03-28 Completed Date: 2002-06-24 Revised Date: 2003-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0323470 Medline TA: Kidney Int Country: United States |
Other Details:
|
Languages: eng Pagination: 1407-15 Citation Subset: IM |
Affiliation:
|
Department of Nephrology and Rheumatology, University Hospital Göttingen, Göttingen, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
4-Chloromercuribenzenesulfonate
/
pharmacology Animals Biological Transport / drug effects, physiology Chlorides / physiology Cytochalasins / pharmacology Kidney Medulla / cytology, metabolism* Kinetics Male Phloretin / pharmacology Phlorhizin / pharmacology Potassium / physiology Rats Rats, Wistar Sodium / physiology Sorbitol / antagonists & inhibitors, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Chlorides; 0/Cytochalasins; 50-70-4/Sorbitol; 554-77-8/4-Chloromercuribenzenesulfonate; 60-81-1/Phlorhizin; 60-82-2/Phloretin; 7440-09-7/Potassium; 7440-23-5/Sodium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Tubular and cellular localization of the cardiac L-type calcium channel in rat kidney.
Next Document: Human uremic plasma increases microvascular permeability to water and proteins in vivo.