| Sonoporation enhances chemotherapeutic efficacy in retinoblastoma cells in vitro. | |
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MedLine Citation:
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PMID: 21273549 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To study the ability of ultrasound (US) and microbubbles (MB) to enhance chemotherapeutic efficacy against retinoblastoma Y79 cells in vitro. METHODS: The experiment was performed in three stages. The authors first compared cell viability of Y79 cells exposed to doxorubicin versus cells exposed to doxorubicin combined with low-intensity, low-frequency US + MB. They then evaluated enhanced cell permeability by studying the intensity of intracellular fluorescence in cells exposed to doxorubicin versus those exposed to doxorubicin with US + MB. Lastly they evaluated the morphologic characteristics of the cells by scanning electron microscopy (SEM) to identify the presence of pores. RESULTS: The Y79 cells exposed to doxorubicin with US + MB showed a significant decrease in cell viability at 72 hours compared with those exposed to doxorubicin alone (P = 0.02). Cells also showed immediate increased permeability to doxorubicin with the addition of US + MB compared with doxorubicin alone, which continued to increase over 60 minutes. SEM did not demonstrate physical pores at the lowest US + MB intensity shown to enhance intracellular doxorubicin fluorescence. CONCLUSIONS: US + MB facilitates the uptake of chemotherapy in retinoblastoma Y79 cells in vitro. This occurs in the absence of visible pores, suggesting a possible secondary mechanism for increased drug delivery. This experiment is the first step toward enhancing chemotherapy with sonoporation in the treatment of intraocular tumors. This technique may lead to more effective chemotherapy treatments with less collateral damage to ocular tissues and may allow reduced systemic dosage and systemic side effects. |
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Authors:
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Nahyoung G Lee; Jesse L Berry; Tom C Lee; Annie T Wang; Scott Honowitz; A Linn Murphree; Neeta Varshney; David R Hinton; Amani A Fawzi |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-06-01 |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 52 ISSN: 1552-5783 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-06-02 Completed Date: 2011-08-19 Revised Date: 2013-05-27 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: United States |
Other Details:
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Languages: eng Pagination: 3868-73 Citation Subset: IM |
Affiliation:
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Doheny Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibiotics, Antineoplastic
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administration & dosage*,
pharmacokinetics Cell Count Cell Membrane Permeability Cell Survival Doxorubicin / administration & dosage*, pharmacokinetics Drug Delivery Systems Drug Synergism Humans Microbubbles* Microscopy, Electron, Scanning Retinal Neoplasms / drug therapy*, ultrasonography, ultrastructure Retinal Pigment Epithelium / drug effects, ultrasonography Retinoblastoma / drug therapy*, ultrasonography, ultrastructure Sonication* Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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EY03040/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibiotics, Antineoplastic; 23214-92-8/Doxorubicin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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