Document Detail

Sonodynamically induced cell damage and membrane lipid peroxidation by novel porphyrin derivative, DCPH-P-Na(I).
MedLine Citation:
PMID:  20651375     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Ultrasonically induced cell damage and active oxygen generation with a novel porphyrin derivative DCPH-P-Na(I), were compared in the same in vitro insonation setup. MATERIALS AND METHODS: Sarcoma 180 cells suspended in air-saturated PBS were exposed to ultrasound at 2 MHz for up to 60 s in the presence and absence of DCPH-P-Na(I). Cell viability was determined with the trypan blue exclusion test. Lipid peroxidation in cell membranes was estimated by measuring the amount of reactive substance produced immediately following the addition of thiobarbituric acid. RESULTS: Significant enhancement of the rates of both ultrasonically induced cell damage and lipid peroxidation was observed in the presence of 2-8 muM DCPH-P-Na(I). Both rates correlated very well. CONCLUSION: The enhancement of both rates with DCPH-P-Na(I) was suppressed by 10 mM histidine. These results suggest that ultrasonically generated active oxygen plays a primary role in the ultrasonically induced cell damage in the presence of DCPH-P-Na(I).
Nagahiko Yumita; Yumiko Iwase; Koji Nishi; Toshihiko Ikeda; Shin-Ichiro Umemura; Isao Sakata; Yasunori Momose
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-08-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  2241-6     Citation Subset:  IM    
Yokohama College of Pharmacy, 601, Matano-cho, Totsuka-ku, Yokohama, Kanagawa 245-0066, Japan.
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MeSH Terms
Free Radical Scavengers / pharmacology
Histidine / pharmacology
Lipid Peroxidation / drug effects*
Mannitol / pharmacology
Membrane Lipids / metabolism*
Mice, Inbred ICR
Porphyrins / pharmacology*
Sarcoma 180 / drug therapy*,  metabolism
Ultrasonic Therapy*
Reg. No./Substance:
0/13,17-bis(1-carboxyethyl)-8-(2-(2,4-dichlorophenylhydrazono)ethylidene)-3-ethenyl-7-hydroxy-2,7,12,18-tetramethylchlorin; 0/Free Radical Scavengers; 0/Membrane Lipids; 0/Porphyrins; 69-65-8/Mannitol; 71-00-1/Histidine

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