| Sonodynamic action of pyropheophorbide-a methyl ester induces mitochondrial damage in liver cancer cells. | |
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MedLine Citation:
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PMID: 21183195 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Sonodynamic therapy with pyropheophorbide-a methyl ester (MPPa) presents a promising aspect in treating liver cancer. The present study aims to investigate the mitochondrial damage of liver cancer cells induced by MPPa-mediated sonodynamic action. Mouse hepatoma cell line H(22) cells were incubated with MPPa (2μM) for 20h and then exposed to ultrasound with an intensity of 0.97W/cm(2) for 8s. Cytotoxicity was investigated 24h after sonodynamic action using MTT assay and light microscopy. Mitochondrial membrane potential (ΔΨm) was analyzed using flow cytometry with rhodamine 123 staining and ultrastructural changes were observed using transmission electron microscopy (TEM). The cytotoxicity of MPPa-mediated SDT on H(22) cell line was 73.00±3.42%, greater than ultrasound treatment alone (28.12±5.19%) significantly while MPPa treatment alone had no significant effect on H(22) cells. Moreover, after MPPa-mediated SDT cancer cells showed swollen mitochondria under TEM and a significant collapse of mitochondrial membrane potential. Our findings demonstrated that MPPa-mediated SDT could remarkably induce cell death of H(22) cells, and highlighted that mitochondrial damage might be an important cause of cell death induced by MPPa-mediated SDT. |
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Authors:
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Jing Xu; Xinshu Xia; Albert Wingnang Leung; Junyan Xiang; Yuan Jiang; Heping Yu; Dingqun Bai; Xiaohong Li; Chuanshan Xu |
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Publication Detail:
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Type: Journal Article Date: 2010-12-03 |
Journal Detail:
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Title: Ultrasonics Volume: 51 ISSN: 1874-9968 ISO Abbreviation: Ultrasonics Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-01-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0050452 Medline TA: Ultrasonics Country: Netherlands |
Other Details:
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Languages: eng Pagination: 480-4 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Rehabilitation Medicine, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China; Department of Ultrasound Medicine, The Eastern District of Sichuan Provincial People's Hospital, Sichuan, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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